MicroED data collection with SerialEM
Abstract
We developed a procedure for the cryoEM method MicroED using SerialEM. With this approach, SerialEM coordinates stage rotation, microscope operation, and camera functions for automated continuous-rotation MicroED data collection. More than 300 datasets can be collected overnight in this way, facilitating high-throughput MicroED data collection for large-scale data analyses.
02 /Specification & Classification
Click any tag to browse matching papers- Assay
- Approach
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Collecting large datasets of rotational electron diffraction with ParallEM and SerialEM
K. Takaba, S. Maki-Yonekura, K. Yonekura·Journal of Structural BiologyPeer-reviewed·Aug 1, 2020
- Optimized cryo-EM data acquisition workflow by sample thickness determination
J. Rheinberger, G. Oostergetel, … C. Paulino·bioRxiv·Dec 1, 2020
- Electron-counting in MicroED
J. Hattne, M. Martynowycz, … T. Gonen·bioRxiv·Jun 29, 2023
- Collection of continuous rotation MicroED Data from Ion Beam Milled Crystals of Any Size
M. Martynowycz, W. Zhao, … T. Gonen·bioRxiv·Sep 24, 2018
- LowDoseWizard - rapid and standardised setup of low-dose cryo-TEM imaging in SerialEM
Simon A. Fromm, Simone Matteï·Acta Crystallographica Section D Structural BiologyPeer-reviewed·Jul 17, 2026
- AutoMicroED: A semi-automated MicroED processing pipeline
S. M. Powell, I. V. Novikova, … J. E. Evans·bioRxiv·Dec 13, 2021