PhenoMS-ML: Phenotypic Screening by Mass Spectrometry and Machine Learning

Protein mass fingerprinting by MALDI-TOF MS in combination with machine learning (PhenoMS-ML) permits the identification of response signatures generated in cell cultures upon exposure to well-characterized drugs. PhenoMS-ML is capable to identify and classify the mode of action of unknown antibacterial agents in wild-type Escherichia coli and Staphylococcus aureus. It allows the sensitive, specific, and high-throughput identification of drug target mechanisms that are difficult to assess by other methods.