flow cytometry

This study uses a multidisciplinary approach to identify age-dependent expression patterns in porcine experimental wounds to improve objective wound age estimation in forensic cases.

This study compares 6-hour and 18-hour stimulation durations for NK cell degranulation assays and finds the 6-hour protocol is more reliable and efficient.

This study identifies CodY regulon and oxidative stress as drivers of rifampicin-induced S. aureus persister formation using single-cell analysis.

This paper introduces phSFC, a method using spectral flow cytometry and phasor analysis to analyze membrane properties in single cells, showing it matches hyperspectral microscopy results.

This study develops L1CAM-CAR T cells that effectively target rhabdomyosarcoma despite low antigen density, showing improved efficacy and safety compared to existing CARs.

The paper shows that glycan-specific B cells undergo class-switching and diversification in germinal centers with age and infection, influencing vaccine design.

The paper characterizes early antigen-specific B cell responses after vaccination, identifying distinct clusters with different contraction dynamics and potential roles in memory B cell formation.

This study develops an in vitro mouse model to study epicardium-derived cardiac fibroblasts using lineage tracing and immortalized cell cultures.

This study compares CyTOF with fluorescent cytometry for detecting intracellular markers, finding CyTOF provides superior resolution for phosphoproteins, transcription factors, and cytokines, enabling better immune cell clustering and discovery of unique populations.

This study shows that adult mice with neonatal-like T cell subsets are more susceptible to Bordetella pertussis but not influenza, highlighting the role of thymic development in immune responses.

This paper identifies systemic reductions in dendritic cell (DC) numbers and activation markers in brain tumor patients, with brain tumors directly contributing to DC defects, and highlights corticosteroid use as a confounding factor.

The paper identifies IL-10-producing pre-Th17 cells in AD-HIES patients that respond to pathogens and support IgE production despite STAT3 mutations.

This study examines neutrophil infiltration patterns in lymph nodes during adoptive T cell therapy in mice, revealing differences between tumor-draining and non-tumor-draining lymph nodes.

This study compares CD4 T cell responses to Plasmodium falciparum and P. vivax malaria, finding similar activation and proliferation but species-specific differences in peripheral Tfh cell subsets and IL-10 production.

This study identifies a functional effector CD8+ T cell program in mice and humans that persists into memory after antigen clearance, driven by CD43 1B11 expression.

This study identifies immunometabolic factors in CD19 CAR T cells that predict long-term response in B-ALL patients and suggests mTOR inhibition as a strategy to enhance anti-tumor activity.

The paper uses full-spectrum cytometry to analyze microalgae and bacteria by their autofluorescence, revealing taxonomic differences and interactions affecting pigment signatures.

This study compares three high-sensitivity flow cytometers for analyzing nanoparticles and fluorescent extracellular vesicles using controlled conditions and different detection methods.

The paper compares T and B cell subpopulations in Four Core Genotypes mice to wild-type mice, finding sex chromosome effects on immune cell populations.

This study investigates how synovial microenvironment stiffness changes during OA progression and how it affects fibroblast and macrophage behavior through mechanosignaling and cell-cell interactions.

The paper introduces CaptureBody, a bispecific antibody for spectral flow cytometry that improves unmixing accuracy by using cells instead of compensation particles.

This paper identifies an RNP/Sm co-positive SLE endotype with myeloid-driven inflammation, hyperferritinaemia, and treatment resistance, suggesting targeted therapies are needed.

This study identifies CD20+ cytotoxic T cells as glucose-responsive, hyperfunctional cells in T2D that correlate with metabolic parameters and may contribute to inflammation.

This study shows that inducing oxidative stress in pancreatic cancer cells creates an effective whole-cell vaccine that enhances anti-tumor immunity and synergizes with PD-1 blockade.

This paper introduces an automated method to estimate the DNA replication cycle duration in E. coli using flow cytometry data without requiring pharmacological treatments.

X-CODE enables multi-platform clonal tracking and spatial phenotyping by combining RNA barcoding with sequencing and imaging technologies.

This study compares ADCC activity of CD56neg and CD56dim NK cells against EBV-type 1 and 2 Burkitt lymphoma cells, finding EBV-T2 cells are more susceptible to direct cytotoxicity.

This study examines age-related differences in T follicular helper cell function and their impact on antibody responses across human development.

This study evaluates BLN-driven cytotoxicity in β and γδ T-cell subsets, finding β T cells show superior cytotoxicity while γδ T cells exhibit stable effector-memory states and reduced susceptibility to activation-induced cell death.

This study identifies persistent immune dysregulation in women with hypertensive disorders of pregnancy years after delivery, linked to increased cardiovascular disease risk.