flow cytometry

The paper compares immune responses to Mycobacterium tuberculosis in African and European donors using CyTOF, finding divergent profiles linked to geographic origin and immune control.

This study identifies and localizes bone marrow ILC2s using dual-reporter mice and combines flow cytometry, transcriptomics, and imaging.

This study examines circulating CD4 regulatory T cells in Parkinson's disease and finds they are largely preserved in phenotype and function, though show reduced HELIOS expression in naive subsets.

The paper develops a 30-marker spectral flow cytometry panel to profile immune cell subsets in African green monkey and baboon PBMCs, focusing on T cell subsets, cytokines, and activation markers.

This paper develops a KIR3DL2-directed CAR T-cell therapy for T-cell lymphomas, showing preclinical efficacy and safety in xenograft models.

The paper identifies immune cell activation patterns associated with rapid clearance of infectious SARS-CoV-2 in both unvaccinated and vaccinated individuals.

The paper develops a 31-marker spectral flow cytometry panel to simultaneously profile functional cytokines, AIMs, and FoxP3+ Tregs in human PBMCs under different conditions.

This study uses serial intravascular labeling to show dynamic changes in decidual NK cell residency and T-bet expression during pregnancy.

The paper benchmarks Quantum Barcoding against mass cytometry in human and rhesus macaque blood, integrating data to reveal conserved and divergent signaling responses across species.

This study examines immune cell changes in obesity and after bariatric surgery, finding preserved type 2 immune cell plasticity and differential immune recovery.

The paper assesses whether HIV-1 infection confers resistance to CTL-mediated cell death and finds no intrinsic resistance, with survival differences attributed to MHC class I downregulation.

The paper shows that glutaminolysis and mitochondrial metabolism are conditionally crucial for B cell differentiation and cytokine signaling, revealing metabolic regulation of plasma cell generation and cytokine receptor signaling.

The paper adapts flow cytometry for field studies of immune cell seasonality in wild bats, revealing seasonal changes in immune cell populations.

This paper introduces a competitive transplantation method using HLA-mismatched human hematopoietic stem cells in humanized mice to study repopulating capacity and track graft dynamics.

This study shows that engineered NKG2C+ NK-like T cells have better antitumor effects and lower cytokine release syndrome risk than conventional CAR-T and CAR-NK cells.

This study investigates how carbonosomes in Pseudomonas aeruginosa affect cell viability and antibiotic sensitivity during nutrient fluctuations and tobramycin exposure.

This study examines immune dynamics during menstruation in mice, identifying monocytes, macrophages, and neutrophils as key players in endometrial repair and highlighting thrombospondin 1 and secreted phosphoprotein 1 as potential therapeutic targets.

This paper develops GAT1508-coated gold nanoparticles for detecting ER+ breast cancer cells through specific binding to GIRK1 channels, validated by flow cytometry and electrophysiology.

The paper shows that Plasmodium sporozoites trigger innate immune responses via cell traversal, which is essential for activating protective CD8+ T cells.

This study shows that HbAS and HbAC red blood cells increase Plasmodium falciparum sexual conversion rates compared to HbAA, suggesting a host genetic factor affecting malaria transmission.

This study shows that migratory type 1 dendritic cells are more effective than lymph node resident type 1 dendritic cells in priming CD8+ T cells in tumor and inflamed tissues.

This paper establishes a human lung explant model to quantify T cell bispecifics' on-target, off-tumor toxicity by measuring T cell activation and epithelial cell killing.

The paper uses FACS to analyze Escherichia coli L-forms and finds they maintain synchronized nucleic acid synthesis despite lacking a cell wall.

This study induces β-cell proliferation in rats using synthetic mRNAs and identifies heterogeneity in cell-cycle dynamics, revealing variability in G1 duration and division completion.

This study shows adenosine impairs T cell function by activating the purine salvage pathway and inhibiting pyrimidine biosynthesis through AMP-mediated suppression of UMP synthase.

This study characterizes T cells in malignant pleural effusions (MPE) and finds they have lower exhaustion and higher cytotoxic potential compared to tumor T cells, suggesting MPE as a better source for TIL therapy.

The paper shows that mouse metaphase chromosomes from different cell types have distinct biomechanical properties, with ESC-derived chromosomes showing conserved force-dependent responses but differing from those of NSCs and preB cells.

This paper introduces a 28-marker flow cytometry panel to profile cancer-associated fibroblasts (CAFs) and reveals their phenotypic and metabolic heterogeneity across different models.

The paper assesses NK cell-mediated ADCC response in chikungunya patients across different phases and finds persistent ADCC in recovered individuals and correlation with antibody levels.