fluorescence / live-cell microscopy

This paper demonstrates that RGB cameras can effectively classify up to six fluorophores in SMLM with high precision, offering a cost-effective alternative to spectral imaging.

This study reveals the structural mechanism of MyD88 oligomerization in innate immune signaling using cryo-EM and high-speed AFM.

The paper evaluates a laparoscopic fluorescence imaging platform to quantify light-triggered drug release in cancer models, showing strong correlation with standard measurements and potential for guided treatment.

The paper adapts a two-photon microscope to detect both visible and near-infrared fluorescence, enabling simultaneous dopamine and calcium imaging in the retina.

This study demonstrates plasmonic enhancement of protein function in living cells by coupling designed nanoparticles to Archaerhodopsin proteins, increasing fluorescence and response speed.

This paper introduces VULCROM, a vacuum-free cryogenic fluorescence microscope that enables high-resolution cryo-SR-CLEM and detailed single-molecule studies in vitrified specimens.

This study examines the dynamic interactions between nucleoli and centromeres in living cells, showing that centromeres move and associate with nucleoli during interphase, with these interactions dependent on rDNA transcription and nucleolar integrity.

This study shows that β-tubulin isotype TBB-4 in C. elegans cilia uses IFT and diffusion to maintain axoneme integrity, with IFT being critical for steady-state length maintenance.

This paper introduces a series of spontaneously blinking dyes with adjustable duty cycles for improved single-molecule localization microscopy in cells and dense structures.

micromorph is a Python toolkit for measuring bacterial morphology from light microscopy data, offering accurate and flexible analysis with a documented API and napari plugin.

This study investigates how chain entropy affects cooperativity during protein unfolding, showing that inter-chain coupling suppresses heterogeneity and promotes coordinated unfolding.

This study shows that the lipid composition of the membrane actively regulates EGFR conformation and signaling, independent of ligand binding, with implications for cancer and normal cell function.

This paper introduces iNTA-F, a method combining interferometric nanoparticle tracking analysis with fluorescence detection to characterize nanoparticles with single-molecule sensitivity.

This study uses live cell FRET microscopy to show that adenovirus E1A's structural ensemble and nuclear/cytoplasmic partitioning change during infection.

OT-Curtains enables real-time observation of DNA-end interactions using optical tweezers and microscopy, demonstrating applications in DNA repair and telomere biology.

This paper introduces GEM-DDM, a method using genetically encoded nanoparticles and differential dynamic microscopy to measure cytoplasmic viscosity in living cells, showing increased viscosity in aggressive colorectal cancer cells.

This study investigates how PKA activity in primary cilia regulates the Hedgehog pathway, showing that ciliary PKA specifically modulates the pathway even when cilia are disrupted.

V-SWITCH is a fluorescent reporter that detects viral infections by switching from OFF to ON fluorescence, enabling live-cell tracking and screening for antiviral strategies.

This study investigates how tricalbin domains regulate membrane contact site organization in yeast through lipid binding and curvature sensing.

CGMD selects a small set of diverse images for fine-tuning pretrained cell segmentation models, achieving performance close to fully supervised methods with minimal annotations.

The paper develops an FCS excitation scan assay to compare fluorescent reporters' brightness and photobleaching in tissue culture and zebrafish embryos, finding mNeonGreen outperforms mEGFP.

The paper investigates human mitochondrial translation initiation on leaderless mRNAs, revealing two pathways involving mtIF2 and fMet-tRNAMet, with implications for regulation by mtIF3.

This study develops and evaluates deep-learning models for amoeba detection in phase-contrast images using annotated datasets and compares their speed and accuracy for real-time applications.

This study maps condensate dielectric permittivity using fluorescence and reveals that membrane interactions depend on permittivity contrast rather than absolute permittivity.

The paper introduces QuEST immunofluorescence microscopy to quantify TCR signalosome stoichiometries in human T cells, revealing a 1:1 ZAP-70:TCR ratio and CD28 recruitment independent of CD80/CD86.

This paper introduces COMET, a drift correction method for SMLM that improves resolution and temporal precision, and demonstrates its effectiveness across multiple SMLM techniques.

Shape2Fate tracks and classifies exocytic and endocytic carriers in live cells using morphology-aware deep learning, revealing pathway-specific exo-endocytic coordination rules.

The paper presents an open-source code to estimate mitochondrial radial localization from fluorescence microscopy images using various markers.

This paper shows curcumin targets the septin ring in fission yeast, delaying cytokinesis and forming a fluorescent ring that co-localizes with septins and the exocyst complex.