fluorescence / live-cell microscopy

The paper introduces a fibre-based structured illumination microscopy (SIM) system that simplifies setup and improves resolution without specialized optics.

The paper shows that striatal value signals track progress toward a goal during planning, supporting a goal gradient rather than full forward-planning models.

This paper uses super-resolution microscopy to show densely packed Ebola virus glycoprotein clusters in the plasma membrane, with high molecular density and cluster size.

This paper introduces Deep3DSIM, an upright 3D-SIM system with adaptive optics for deep tissue imaging, achieving high-quality imaging up to 130 micrometers in Drosophila brain.

CLEM-Reg automates 3D alignment of vCLEM datasets, achieving sub-micron correlation of fluorescent signals with EM structures and enabling identification of TGN46-positive transport carriers.

This study shows that CHMP2A is essential for the ordered assembly of ESCRT-III proteins during cytokinetic abscission, revealing a hierarchical organization of these subunits.

The paper shows that wound healing in Drosophila involves reversible chromatin-lamina dewetting triggered by mechanical changes, leading to cellular contraction and tissue repair.

The paper develops a DNA labeling technique to study chromatin mobility and organization during neural differentiation, finding reduced chromatin mobility and increased compaction in differentiated cells.

The paper introduces a multispectral imaging approach for live-cell imaging, enabling accurate unmixing of low signal-to-noise ratio data at video rates.

This paper investigates how varying carotenoid conjugation lengths in bacterial LH2 complexes affect exciton dynamics and singlet-triplet annihilation, revealing that lower carotenoid triplet energy improves BChl triplet quenching.

This paper develops a framework to enhance the signal-to-noise ratio in quantitative fluorescence microscopy by optimizing camera parameters and microscope settings.

This study tracks DNA-origami nanostructures in zebrafish embryos using imaging and single-cell RNA sequencing to understand their biodistribution and clearance mechanisms.

The paper shows polyaneuploid prostate cancer cells under hypoxia exhibit enhanced motility and aerotaxis driven by HIF-1α and RhoA, enabling invasion and intravasation.

Mice infer stimulus-side priors from recent choices; prior signals are decodable pre-stimulus in 24.0% to 30.2% of 242 brain regions, including early visual and motor areas.

The paper investigates NPQ development in aspen and Arabidopsis npq mutants under photoinhibitory and non-photoinhibitory conditions, finding that sustained quenching can occur independently of photoinhibition.

This paper introduces an AI system that forecasts future embryo morphology in time-lapse videos using Convolutional LSTM layers, showing accurate predictions for cleavage and blastocyst stages.

This study uses live-cell single-molecule fluorescence microscopy to investigate MYO7A trafficking in stereocilia, revealing its dimeric form and movement restrictions mediated by plasma membrane and F-actin interactions.

Smartphone-video biomechanics (OpenCap) reproduced four timed function tests (r > 0.98) and separated facioscapulohumeral from myotonic dystrophy better than test times (balanced accuracy 82% vs 50%; 129 participants).

This paper introduces an enhanced mountain climbing search algorithm for fast and accurate autofocus in high-resolution fluorescence microscopy, achieving up to 200-fold improvement in precision over existing methods.

The paper introduces event-triggered MINFLUX microscopy to efficiently capture rare cellular events by triggering MINFLUX acquisition based on real-time confocal imaging analysis.

The paper introduces NitrOFF, a fluorescent biosensor for real-time nitrate detection in mammalian cells, showing it responds to nitrate with a turn-off fluorescence mechanism.

This study investigates endocytic internalization in Aspergillus nidulans, revealing Dip1's role in priming Arp2/3 for actin filament synthesis and identifying Dip1-independent mechanisms for seed filament formation.

This study uses supervised machine learning to combine 3D, multispectral, and thermal imaging data for improved detection of root rot in hydroponic parsley.

This study identifies distinct shelterin subcomplexes at telomeres, showing TRF1-TIN2-TPP1-POT1 and TRF2-RAP1 occupy non-overlapping sites with different dynamics.

This paper develops a method to fluorescently label and track non-repetitive genomic loci in live human cells, showing gene-specific chromatin dynamics independent of transcription.

The paper applies a quantum-classical model to explain Bicoid morphogen gradient dynamics and its interpretation by the Hunchback gene in Drosophila embryos.

The paper develops a pipeline for high-resolution imaging and analysis of whole mouse glomeruli, revealing novel aging and disease-related phenotypes.

This paper introduces a Bayesian nonparametric framework for analyzing single-molecule fluorescence data, demonstrating its effectiveness through synthetic and experimental data.

This study uses smFRET to analyze conformational dynamics of FicD's TPR motifs, showing equilibrium between TPR-in and TPR-out states influenced by ATP and BiP.