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The paper develops a red fluorescent sensor, chILI, for detecting cAMP in living cells through intensity and lifetime imaging, demonstrating its use in mouse brain neurons.

This study quantifies spatio-temporal image degradation from motion blur in fluorescence microscopy using SSNR and simulations, showing resolution loss up to 40% for 90-degree motion direction changes.

This paper introduces TomoFLIM, a high-speed multiphoton FLIM technique that enables rapid, accurate imaging of dynamic biological processes with compression ratios over 90%.

The paper introduces a method for live-cell super-resolution microscopy using fluorogenic HaloTag ligands and BF dyes, enabling single-molecule localization and diffusivity mapping of proteins.

This paper introduces a deep learning method called AUTO-FLI for 3D fluorescence lifetime imaging in deep tissues, showing it can accurately reconstruct intensity and lifetime data in scattering media.

This paper demonstrates that self-blinking dye JF635b improves nanobody performance in single-molecule localization microscopy, enabling reliable imaging across multiple modalities with high precision.

The paper demonstrates a method to multiplex FLIM using small molecule probes modified with bioorthogonal chemistry to achieve distinct fluorescent lifetimes without transfection.

This paper introduces RSpFAST, a non-covalent chemogenetic platform for fluorescence imaging, and demonstrates its dual behavior as a stable label and a reversible photoswitcher.

This paper develops MSCNet, a cross-modal generative model for reconstructing and restoring prostate MRI sequences, showing improved image quality and diagnostic performance in multicentre validation.

This paper introduces enhanced 2D-SIM to reduce reconstruction artifacts and improve resolution in thick samples using optimized coarse and fine patterns.

The paper develops red-shifted near-infrared fluorescent proteins for deep-tissue SWIR imaging, showing improved signal and imaging depth in vivo.

This paper introduces a photon pooling technique for faster single-cell FLIM analysis without machine learning, achieving single-cell metabolic data every second and 15x faster mosaic acquisition.

The paper introduces a method to measure temperature with high precision and resolution using fluorescence lifetime microscopy with Cy5 dye, enabling simultaneous multi-channel imaging.

This paper introduces IP-FLIM, a method that improves fluorescence lifetime imaging by reducing noise and increasing contrast-to-noise ratio.

This paper introduces LUNAR, a self-supervised neural-physics learning framework for 3D localization microscopy that improves robustness to optical aberrations and high-density molecule localization.

This paper introduces a series of spontaneously blinking dyes with adjustable duty cycles for improved single-molecule localization microscopy in cells and dense structures.

This paper introduces a method to enable continuous single-molecule tracking beyond photobleaching limits by designing reversible probe exchange kinetics.

This paper provides a protocol for building and characterizing an oblique plane microscope (OPM) to enable fast, multi-color, high-resolution volumetric fluorescence imaging with reduced phototoxicity.

The paper characterizes MINFLUX imaging performance over extended durations using DNA origami and corrects residual drift, achieving sub-2 nm precision without loss of site precision.

This paper introduces RIED, a super-resolution imaging method using electrochemiluminescence, achieving high sensitivity and resolution for intracellular structures.

This paper introduces caging-group-free photoactivatable fluorophores with far-red emission for improved super-resolution microscopy.

This paper introduces SP-STORM, a method combining single molecule photoswitching and spectral phasor analysis to achieve high-throughput, multiplexed super-resolution imaging of subcellular structures.

This paper introduces engineered magneto-sensitive fluorescent proteins that enable quantum sensing in living cells through optically detected magnetic resonance.

This paper introduces a macroscope for wide-field fluorescence imaging in microfluidic devices, achieving high sensitivity and temporal resolution over a large area.

This paper introduces a real-time fluorescence lifetime imaging method using single-snapshot acquisitions, demonstrating its application in biomedical scenarios from microscopic to macroscale.

This paper introduces a single-objective lattice light sheet microscopy platform for improved 3D single-molecule super-resolution imaging in scattering samples.

The paper develops a biocompatible fluorescent probe for tubulin labeling in live and fixed cells, enabling high-resolution imaging with minimal functional disruption.

The paper develops all-optical strategies to reduce photo-bleaching in reversibly switchable fluorescent proteins, enhancing imaging resolution and duration.

This paper accelerates fluorescence lifetime imaging (FLIM) using single-objective light-sheet microscopy, achieving faster and less phototoxic imaging of live multicellular systems.

The paper introduces FLUMMI, a peptide marker for imaging internal membranes in Plasmodium without affecting viability, and shows its compatibility with live-cell and expansion microscopy.