Sperm and Testicular Function

Analysis of sperm processing and cryopreservation procedures in the common marmoset (Callithrix jacchus) to reduce fragmentation and maximize total and progressive motility

The paper optimizes sperm processing and cryopreservation methods in common marmosets to improve motility and reduce DNA fragmentation for IVF applications.

Nanoscale imaging resolves canonical topology and intracellular dynamics of SUN5/SPAG4L during mammalian spermiogenesis

This study uses advanced imaging techniques to clarify SUN5's localization and dynamics during spermiogenesis, resolving previous controversies about its structure and function.

In Step, the sole Drosophila TENT5 poly(A) polymerase, is required for spermatid elongation and individualization

This study identifies the Drosophila TENT5 poly(A) polymerase, Isep, as essential for spermatid elongation and individualization, with its function independent of poly(A) polymerase activity.

Single-nucleus transcriptomics and phosphoproteomics identify misregulated sperm components in mice carrying a selfish supergene

The paper identifies misregulated sperm components in mice with a selfish supergene using single-nucleus transcriptomics and phosphoproteomics, revealing dysregulated phosphorylation and gene expression related to sperm motility.

Ether-lipid deficiency remodels the testicular interstitium beyond spermatogenic arrest in Gnpat-knockout mice

This study shows that Gnpat-knockout mice exhibit testicular interstitial remodeling and germ-cell loss due to ether-lipid deficiency, with no DNA-damage checkpoint activation.

Stepwise Proteinase K Digestion Improves Genomic DNA Yield and Purity from Goat Semen: A Comparative Pilot Study of Five Extraction Protocols

This study compares five DNA extraction protocols from goat semen, finding that Modified Protocol 2 provides the highest DNA yield, purity, and PCR success.

A Dataset of Human Sperm Microscopy Videos for Detection, Tracking, and Motility Parameter Analysis

The paper introduces HSMV, a dataset of human sperm microscopy videos for detection, tracking, and motility analysis, with annotated and unannotated clips for algorithm benchmarking.

Mitochondrial and metabolic plasticity distinguish stem cells from progenitors in the male germline

This study compares mitochondrial and metabolic properties of stem and progenitor cells in the male germline, finding that stem cells exhibit metabolic plasticity and latent oxidative capacity.

Adenosine refines the balance of spermatogonial proliferation and differentiation

This study shows adenosine promotes spermatogonial differentiation and regulates the balance between proliferation and differentiation in mice.

Small-molecule modulators of HIPK4 activity and proteostasis

This study identifies cyanoquinoline-based HIPK4 inhibitors with nanomolar potency and reveals their cell-type-specific effects on HIPK4 proteostasis and autophagy.

Mitochondrial Calcium Uptake Promotes Mitochondrial Activation and Hyperactivated Motility During Human Sperm Capacitation

This study shows that mitochondrial calcium uptake via CatSper and MCU is essential for hyperactivated motility during human sperm capacitation, similar to mouse sperm.

A genetic screen to identify novel interactions of the phosphatidylinositol phosphate signaling pathway in Drosophila spermatogenesis

The paper identifies genetic interactions of the phosphatidylinositol phosphate signaling pathway in Drosophila spermatogenesis through a sensitized genetic screen.

Amyloid-mediated RNA uptake by sperm for embryonic delivery

This study identifies amyloid fibrils in seminal fluid that bind and deliver RNA to sperm and the zygote during fertilization.

A low concentration of a sustainably obtained blueberry extract improves the post-thawing motility of cryopreserved bull spermatozoa

Calcium-activated chloride channels, rather than the voltage-gated ones, regulate sperm capacitation

This study identifies calcium-activated chloride channels (CaCCs) as key regulators of sperm capacitation, showing their greater impact compared to voltage-gated chloride channels.

Sperm development requires TMC5-dependent mechanoresponsive calcium entry

This study identifies TMC5 as essential for mechanoresponsive calcium entry during spermiogenesis, linking mechanical cues to sperm development and male infertility.

Seminal extracellular vesicles from boar AI doses contain fertility-predictive protein and miRNA cargo and improve sperm physiology

This study identifies fertility-predictive protein and miRNA cargo in seminal extracellular vesicles from boar AI doses and shows their functional impact on sperm physiology.

Paternal diet shapes paternal and maternal transcriptomes in the early embryo through parallel sperm-associated mechanisms

The paper shows that paternal diet affects both paternal and maternal transcriptomes in early embryos through distinct sperm-related mechanisms.

Mapping the human sperm proteome across compartments, donors and individual cells

This study maps the human sperm proteome across compartments, donors, and individual cells, revealing conserved and variable proteins and linking mitochondrial dysfunction to male infertility.

Transcriptome profiling identifies the chromatoid body as a dynamic center for RNA surveillance and transcript sorting during spermatogenesis

The paper identifies the chromatoid body as a dynamic RNA sorting center in spermatogenesis, revealing its role in RNA surveillance and transcript regulation through sequence and structural features.

LRRC37A is essential for acrosomal ion homeostasis and male fertility

This study identifies LRRC37A as critical for acrosomal ion homeostasis, showing its loss leads to male infertility and acrosomal enlargement.

Effects of Deficient Glycosylation and Deglycosylation on Sperm Condition in Zebrafish (Danio rerio)

This study examines the effects of glycosylation and deglycosylation disorders on zebrafish sperm quality, showing reduced concentration, motility, and membrane integrity in mutant fish.

Mammalian spermatogenesis at single-cell resolution downstream of genetic perturbations in meiotic double-strand break repair

Single-nucleus RNA+ATAC profiling of 32 mice shows asymmetric PRDM9 binding drives asynapsis, pachytene cell loss, aneuploidy and multi-megabase crossover redistribution, tuned by a chromosome 15 locus.

Effect of seminal plasma extracellular vesicles in post-thaw functional parameters of cryopreserved ram sperm

This study shows that adding seminal plasma-derived extracellular vesicles to ram semen cryopreservation improves membrane lipid organization and intracellular calcium levels, and alters sperm movement patterns.

Surface N-acetylglucosamine dynamics in bovine spermatozoa: from epididymal transit to oviductal epithelial cell binding

This study investigates how surface N-acetylglucosamine (GlcNAc) changes in bovine sperm during epididymal maturation and its role in binding to oviductal epithelial cells.

A single-nucleus multiomic and spatial atlas of gene regulation in human spermatogenesis

This study creates a single-nucleus multiomic and spatial atlas of human spermatogenesis, linking gene regulatory networks to infertility-associated genetic variation and disease perturbations.

Male Age and Sexual Maturity: Lipopolysaccharide-induced tumor necrosis factor influences sperm quality and reproduction in Anopheles culicifacies

This study investigates age-related changes in male reproductive capacity in Anopheles culicifacies, identifying AcLITAF6 as a key regulator of sperm quality and male fertility.

Sperm-specific fertility factors SMZ-1/2 promote FB-MO biogenesis and MSP filament assembly in C. elegans

This study identifies SMZ-1/2 as PDZ proteins that initiate MSP filament assembly and FB-MO biogenesis in C. elegans sperm by coupling kinase signaling to organelle formation.

Exploratory metagenomics of bacterial diversity in semen from indonesian native roosters supplemented with curcumin and penicillin-streptomycin

This study evaluates how curcumin and penicillin-streptomycin affect the diversity and structure of the chicken semen microbiome using 16S sequencing.

Early life stress affects the transcription and chromatin accessibility of spermatogonial cells

This study shows early-life stress alters the transcriptome and chromatin accessibility of mouse spermatogonial cells, with molecular signatures persisting into adulthood.