Bacterial Genetics and Biotechnology

The paper introduces pTRIP, a new integrative plasmid for Listeria monocytogenes that allows stable gene integration without antibiotic selection.

This study identifies a lipid cue that drives the subcellular localization of a bacterial transmembrane protein, ShfA, by binding to undecaprenyl phosphate.

This study shows RNase J2 in B. subtilis acts as a specificity factor for RNase J1, enhancing its degradation of mRNAs and influencing biofilm formation and swarming.

The paper shows that cell death affects the relationship between DNA replication-based estimates and population size changes in bacteria.

The paper identifies CdgKA and CdgSHA as a complex two-component system that regulates c-di-GMP degradation and cell size in Anabaena sp. PCC 7120.

This study identifies PspA's role in maintaining membrane potential and supporting NADH dehydrogenase function in mycobacteria through interactions with PspM and menaquinone.

This study shows that bacterial dormancy lag time is controlled by proteostasis, with DnaA sequestration in aggregates delaying replication initiation and extending dormancy.

This study defines the interactions among mycobacterial mRNA degradation enzymes and identifies the functional roles of RNase E's intrinsically disordered regions in degradation processes.

This study shows that silencing racR in E. coli triggers stress responses linked to prophage excision, revealing mechanisms of survival and cell death under lethal conditions.

This study identifies TFB2, a TFIIB paralog, as a key regulator of cyclic transcription in Sulfolobus acidocaldarius, linking it to cell cycle progression without CDKs.

This study shows that acetylation of CRP K52 activates AR3, leading to changes in gene expression that affect sugar utilization and virulence in V. cholerae.

This study reveals that Ignicoccus hospitalis uses a contractile CdvA-ESCRT-III ring to constrict its inner membrane, leading to multi-cytoplasmic clusters and subsequent release of progeny cells through outer membrane bursting.

This study identifies SMU_393 as a homolog of RocS in S. mutans through phenotypic profiling of essential genes, revealing its role in chromosome segregation and cell cycle control.

This study shows cyclic di-AMP affects cell division and penicillin susceptibility in Streptococcus pneumoniae by regulating division machinery and cell wall composition.

This study defines the S. aureus CymR-dependent and -independent sulfur starvation responses using chemically defined growth conditions and identifies key sulfur sources and their regulatory mechanisms.

The paper identifies an Esx secretion system in Actinomycetota that defends against parasitic Patescibacteria by sensing epibionts and triggering a transcriptional response.

The paper identifies a stress-induced membrane insertion mechanism that activates the BepA metalloprotease by displacing its autoinhibitory plug, linking membrane association to protease function.

This study identifies Rhs2 as a dual-kingdom T6SS effector that targets bacterial and fungal cells through distinct mechanisms, revealing how a single effector can act against different cellular architectures.

This study identifies pyoverdines as metallophores involved in lanthanide uptake in Pseudomonas putida, expanding their role beyond iron scavenging.

This study examines the conservation of the Atc protein system's functional traits across bacteria, showing preserved core interactions despite sequence variability.

The paper assesses how inner-membrane transporter genotypes affect susceptibility to non-lytic antimicrobials and bacterial fitness in Escherichia coli.

This study identifies two sRNAs in Staphylococcus aureus that regulate zinc homeostasis, showing their role in Zn-sparing responses and adaptation to metal limitation.

This study identifies MxtR/ErdR as a central regulator of short-chain fatty acid metabolism in Pseudomonas alloputida by coordinating acetate and propionate metabolism through direct regulation of the methylcitrate cycle and other pathways.

The paper identifies PusCD1 and PusCD2 complexes in Flavobacterium johnsoniae that enable growth on phosphatidylinositol, revealing a new mechanism for phosphorus utilization in plant-associated Bacteroidota.

This study identifies YumC as essential for isoprenoid and peptidoglycan biosynthesis in B. subtilis by linking it to electron transfer to the IspG enzyme.

This study identifies genes critical for anaerobic maintenance in Pseudomonas aeruginosa using RB-TnSeq, revealing flagellar rotation as an energy drain.

The paper identifies how the Pil-Chp system regulates type IV pilus dynamics in Pseudomonas aeruginosa by modulating cAMP levels and affecting fiber length, count, and extension rate.

This study elucidates the signaling mechanism of the E. coli Aer receptor by analyzing its redox-dependent structural changes and their impact on kinase domain conformation.

The paper expresses MreB isoforms from Haloplasma in a synthetic bacterium to study motility, finding distinct movement patterns and force directions.

This study identifies DksA as a key regulator of the stringent stress response in K. pneumoniae, linking it to antibiotic resistance, virulence, and gastrointestinal colonization.