Mosquito-borne diseases and control

This study knocks out female-specific flight muscle genes in Aedes aegypti mosquitoes, resulting in flightless females and flying males, offering a potential female-specific population control strategy.

The paper uses CRISPR screening in porcine cells to identify host factors essential for Japanese encephalitis virus replication and entry.

The paper uses genomic data to show multiple ZIKV introductions and cryptic transmission in Colombia, with most transmission occurring locally.

The paper evaluates the effect of defective interfering particles on dengue virus within-host dynamics using population of models to estimate their therapeutic potential.

This study shows that genetic diversity in Collaborative Cross mice affects ZIKV infection outcomes, including viral replication and disease severity, independent of Oas1b.

This study uses high-resolution proteomics to identify 26 differentially expressed proteins that can predict Zika virus infection with 72% accuracy using a machine learning model.

The paper introduces a high-throughput tool to detect arboviruses in mosquitoes from Zika-endemic regions, showing its effectiveness in identifying circulating viruses.

The paper updates the Zika virus genotype classification by analyzing 414 genomes and identifying three main clades with further sub-clades, using maximum likelihood trees.

The paper identifies co-circulation of DENV-1 and DENV-3 during the 2019 dengue epidemic in Tanzania, with DENV-1 being dominant and co-infection observed in two samples.

This paper develops an LSTM-based dengue forecast model using monthly case and meteorological data, showing improved accuracy over existing models.

The paper updates the phylogeny of Chikungunya virus and identifies lineage-specific RNA architecture in its untranslated regions.

The paper identifies novel RNA viruses in Anopheles mosquitoes from Senegal and Cambodia, revealing virome diversity and potential interactions with small RNA pathways.

This study identifies a new DENV2 lineage replacement in Brazil using portable genomic surveillance, detecting its introduction from the Caribbean in 2014.

This study evaluates the antigenicity of recombinant ZIKV envelope domain III protein expressed in E. coli using antisera from mice immunized with ZIKV particles, showing cross-reactivity with a different ZIKV strain.

The paper reports natural infection of Zika and Yellow fever viruses in sylvatic mosquitoes in Brazil, including vertical transmission of Zika, suggesting potential for sustained transmission in forested areas.

This paper develops a hybrid ARIMA-NNAR model to forecast dengue cases in three regions, showing improved accuracy over existing methods.

DEN-IM is a workflow for analyzing DENV sequencing data from shotgun and targeted metagenomics, enabling CDS inference, serotype/genotype identification, and phylogenetic tree generation.

The paper analyzes Musashi binding elements in Zika and related Flavivirus 3’UTRs using a thermodynamic model to show ZIKV MBEs are maximally accessible and linked to neurotropism.

The paper maps the genome-wide association of capsid proteins with viral RNA in Dengue and Zika viruses, showing non-uniform binding patterns and sequence preferences.

The paper examines JEV genotypes in Chinese mosquitoes, finding G1 as the dominant genotype, with G3 and G5 present but no G2 or G4.

The paper uses comparative genomics to analyze 3’UTR RNA structures in flaviviruses, identifying conserved xrRNAs and duplicated structures across different viral groups.

This paper describes an optimized impression cytology method to obtain viable ocular cells from babies with Congenital Zika Syndrome, enabling transcriptomic and proteomic analyses.

The paper uses genome-wide SNPs to trace the origin of pyrethroid-resistant Aedes aegypti incursions at international ports, identifying Bali and Pacific Islands as key sources.

The paper identifies domains in the Sindbis virus TF protein that regulate its palmitoylation state and its incorporation into virions.

This paper demonstrates that the 5′-UTRs of Dengue and Zika viruses have IRES activity, enabling translation without a cap, and can initiate infection in mammalian and mosquito cells.

This study integrates transcriptomic, proteomic, and genomic data from CZS neonates to identify collagen gene reductions and associated polymorphisms, linking them to neuronal migration defects and CZS susceptibility.

This study generates Ae. aegypti mosquitoes engineered with synthetic miRNAs to reduce ZIKV infection, dissemination, and transmission.

This study infects olive baboons with Zika virus during mid-gestation and observes fetal cerebral cortical injury and fetal death, providing a new non-human primate model for studying Congenital Zika Syndrome.