Microbial Natural Products and Biosynthesis

This study optimizes heterologous expression and fermentation conditions to produce ikarugamycin at high yields, making it more affordable for biomedical research.

The paper identifies and characterizes the diversity of substrates and partner proteins for multinuclear non-heme iron-dependent oxidative (MNIO) enzymes, revealing new insights into their role in bacterial copper homeostasis and potential broader stress responses.

This study identifies cyclic lipodepsipeptides as the molecular basis of haemolytic activity in Chromobacterium haemolyticum and shows their role in biofilm formation and swarming.

This paper characterizes cyanochelin B, a photolytic siderophore from cyanobacteria, showing it can both monopolize iron and release it under UV light, depending on environmental conditions.

This paper identifies cysimiditides, a new class of RiPPs with a Zn-tetracysteine motif and aspartimidylation, showing their biosynthesis and structural features.

The paper reports the draft genome sequence of Delftia tsuruhatensis IICT-RSP4, an uricase-producing soil bacterium, revealing its potential for antimicrobial, anticancer, and antimalarial secondary metabolites.

This study shows that surfactin represses subtilosin A production in Bacillus subtilis, revealing an ecological interaction between secondary metabolites.

This paper uses mutasynthesis to generate stambomycin derivatives by engineering a bacterial strain and leveraging a promiscuous acyl transferase domain, resulting in new analogues with similar or reduced bioactivity.

This study shows that surfactin production by Bacillus subtilis enhances its establishment success in synthetic bacterial communities.

PanBGC provides a framework to analyze biosynthetic gene clusters across microbial genomes, classifying genes into core, accessory, and unique categories and quantifying diversity through openness metrics.

The paper characterizes pulcherriminic acid biosynthesis in yeast using a heterologous system, showing that K. lactis genes produce faster acid than Metschnikowia genes and that Pul3 mediates both uptake and export of the compound.

This study investigates the acyl group selection mechanism in fungal hrPKS condensing regions through in vitro reconstitution and mass spectrometry, revealing substrate recycling and kinetic parameters as key factors.

The paper describes how GNAT enzymes iteratively acylate lasso peptides to produce lipolasso, expanding the diversity of ribosomal lipopeptides.

This paper discovers a new class of cancer immunogenic chemotherapeutic leads called aspercyclicins through a high-throughput screening platform and structural elucidation techniques.

This paper identifies pseudovibriamides A and B as bacterial signaling molecules that modulate transcription and affect motility in Pseudovibrio brasiliensis.

This study characterizes SidF, a transacetylase in Aspergillus fumigatus, revealing its structure and function in siderophore biosynthesis and its role in complementing SidL during iron deficiency.

The paper investigates a gene cluster that produces both enantiomerically pure and racemic metabolites through a stereodivergent pathway involving a dihydrosydnone N-oxide intermediate.

The paper shows that inactivating Teazeled receptors in human leukocytes increases production of antimicrobial and anticancer compounds, with enhanced activity and stability.

The paper designs an engineered biosynthetic pathway in E. coli to produce (+)-brevianamides A and B, achieving a 46% yield with a 92:8 diastereomeric ratio.

This study identifies YtkR4 and YtkR5 as essential deoxyribonucleases for repairing DNA damage caused by yatakemycin, supporting their role in base excision repair.

The paper identifies sphagnum-associated fungi that produce antitubercular agents inducing thiol stress in Mycobacterium tuberculosis.

This paper identifies bufferins, a widespread family of ribosomal peptide metallophores that help bacteria adapt to copper stress by sequestering excess copper ions.

This study uses kinetic assays to show that reaction rates of isolated modifications can predict the final ring pattern but not the order of modification in ProcM variants.

The paper identifies and partially reconstitutes the biosynthetic pathway of hericenones in Hericium erinaceus using heterologous expression in Aspergillus oryzae.

The paper isolates and genomes bacteria from CCA to identify antimicrobial compounds, finding 100 putative BGCs potentially involved in novel antimicrobial biosynthesis.

The paper uses pattern-based genome mining to discover indanopyrrole A, a novel antibiotic from a marine Streptomyces, and links it to an orphan biosynthetic gene cluster.

This study presents 121 new Bacillales genomes, identifies 1,176 biosynthetic gene clusters, and provides open-access data to enhance research on secondary metabolite production.

The paper redefines the function of EshA as a protein shell encapsulating 2-MIB synthase, showing it is a cargo-loaded nanocompartment for secondary metabolite biosynthesis.

This study investigates the substrate selectivity of the FbsH adenylation domain in fimsbactin biosynthesis, identifying structural modifications that expand substrate tolerance and reveal downstream selectivity bottlenecks.