Lipid Membrane Structure and Behavior

This study investigates how cholesterol influences CD81 tetraspanin conformational changes and membrane deformation through molecular dynamics simulations.

The paper demonstrates a method to synthesize membrane-bound vesicles using a cell-free system by coupling fatty acyl-CoA ligase expression with lipid precursors.

This paper optimizes the cDICE method to produce high-quality, defect-free GUVs with efficient encapsulation of complex biochemical systems.

This paper introduces a microfluidic platform for producing surfactant-free, additive-free biomimetic giant unilamellar vesicles (GUVs) using PDMS-based devices.

This paper describes a chemoenzymatic method using bacterial fatty acid synthase to generate phospholipid membranes from acetyl-CoA and malonyl-CoA precursors.

This study uses explicit solvent simulations to reveal how PTEN interacts with phosphoinositide-enriched membranes, elucidating its mechanism of activation and membrane anchoring.

The paper identifies CRAC and CARC motifs in RTK transmembrane domains, showing cholesterol interaction with TRKB's CARC motif and its effect on BDNF binding.

The paper benchmarks MD models of phospholipid bilayers against NMR data, finding that CHARMM36 and Slipids better capture conformational dynamics than other force fields.

This paper introduces a method to detect permeation events in lipid bilayer simulations, showing permeation frequency and time are independent of force fields and cholesterol reduces permeation.

This study develops a live-cell method to assess keratinocyte differentiation using membrane packing order as a marker, applicable at single-cell and high-throughput levels.

This study investigates how CD81 senses cholesterol and how this affects its function as a hepatitis C virus receptor, finding that cholesterol binding regulates CD81's conformation and receptor activity.

This paper introduces lipid sponge droplets as programmable synthetic organelles that can sequester proteins, enhance reaction rates, and control enzymatic reactions through reversible and optically controlled interactions.

The paper introduces an agent-based model to simulate molecular aggregation at the plasma membrane, showing it can recapitulate observed distributions using simple rules.

This paper demonstrates DNA-functionalized vesicles that enable targeted fusion and controlled protein expression in vitro.

This study uses microfluidic traps to deform GUVs into non-spherical shapes and observes how FtsZ organization changes in response to membrane geometry.

This study shows that the lipid environment affects dopamine transporter function, specifically influencing amphetamine response in mice.

The paper presents evidence that nanoelectrospray can enable membrane protein complexes to self-assemble in lipid bilayers.

This paper introduces NMFk, an unsupervised machine learning method, to analyze lipid phase separation in MD simulations, identifying roles of lipids, nano-domains, timescales, and physical features.

RaftProt V2 improves a database of lipid raft-associated proteins by enhancing search capabilities, visualization, and integration of datasets across cell/tissue types and UniRef/Gene levels.

This study investigates how actin cortex remodeling affects lipid domain organization by using reconstituted actin cortices and phase-separated lipid membranes.