Microtubule and mitosis dynamics

This study identifies unique mitotic features in Paradiplonema papillatum using fluorescence microscopy and YFP-tagging, revealing novel structures like metaphase rings and potential kinetochore localization.

The paper uses cryo-EM to identify an early intermediate state in dynein activation by LIS1, termed Pre-Chi, and shows its functional importance through mutational analysis.

The paper shows Nde1 enhances Lis1 binding to autoinhibited dynein, facilitating Phi conformation opening through a novel intermediate state.

This study shows that biomechanical stress in embryonic neural progenitors affects mitotic fidelity through cortical actin and tension.

The paper identifies a gene that promotes viability in cells with centrosome amplification and multipolar division by positioning centrosomes at the plasma membrane.

The paper identifies a beta-hairpin motif in kinesin-2 that regulates motility by sequestering motor domains, revealing its role in activation and coordination with dynein.

This paper challenges the conventional view of trisomy 12 origin in iPSCs by showing it arises de novo in diploid cells through anaphase bridging and micronuclei formation, leading to rapid dominance of trisomic cells.

The paper identifies Dad1 as a potential substrate of Hrr25 kinase in yeast through yeast two-hybrid screening and structural analysis.

This study reveals that cytokinesis in C. elegans embryos occurs through membrane rupture and independent extension of sister membranes, challenging the classical model of continuous membrane invagination.

This study identifies hSpindly as a dynamic modulator of SAC activity via the RZZ pathway, independent of the KBB pathway, and highlights its role in drug resistance.

The paper identifies A-C linker proteins and shows their role in centriole cohesion, duplication, and structure.

This study identifies spastin's role in regulating spindle dynamics and chromosome segregation during mitosis in Trypanosoma brucei.

The paper identifies Unkempt as a key RNA-binding protein promoting local translation at centrosomes during Plk4-induced centriole overduplication.

The paper investigates how multi-kinesin clusters induce mechanical stress leading to microtubule breakage, estimating a lower rupture force than previous in vitro studies.

The paper shows how LC8 converts the disordered KANK1 linker into a rod-like structure through cooperative multivalency, resolving a cellular architecture paradox.

This study identifies a cell cycle-dependent phosphorylation mechanism that regulates M18BP1 binding to CENP-A nucleosomes, controlling CENP-A assembly during interphase.

The paper determines the structure of the chromosomal passenger complex targeting module bound to phosphorylated nucleosomes, revealing a pivot-tether mechanism for Aurora B localization.

This study uses the AID system with CRISPR/Cas9 to acutely degrade nucleolin in TNBC cells, revealing its role in cell cycle progression and division, and showing that its abrogation enhances the efficacy of mitotic inhibitors.

This study develops a coarse-grained model to simulate microtubule dynamics, validates it against experimental data, and demonstrates its effectiveness in simulating microtubule twist driven by motor proteins.

This study characterizes mitotic chromosome mechanics and shows the mitotic chromosome periphery (MCP) provides force-damping properties and structural constraints.

This study uses cryo-EM to reveal how microtubule association induces an Mg-free apo-like ADP pre-release conformation in kinesin-1, independent of tail interactions.

This study uses multiscale modeling to show that microtubule plus-end tips bend outward into curved structures regardless of GTP hydrolysis state, challenging existing models linking curvature to nucleotide state.

This study identifies how Orientia tsutsugamushi uses ScaC to recruit BICD2 for dynein-based motility, revealing a mechanism for bacterial transport within host cells.

This study shows that K. marxianus has a single centromeric nucleosome that mediates attachment to one microtubule, similar to S. cerevisiae.

The paper shows centrosomes organize Polo kinase (Cdc5) to adapt to DNA damage by phosphorylating SPB components, bypassing cell cycle arrest.

This study identifies Anapc5 and Anapc7 as genetic modifiers of KIF18A function in fertility and mitotic progression by analyzing mouse strain differences and cell line models.

The paper develops a computational model of PRC1 crosslinked microtubule pairs to show that braking and coasting modes depend on lateral separation, with braking reducing sliding speed more effectively.

The paper shows that CDK4/6 inhibitor-treated cells that evade senescence experience mitotic errors and aneuploidy due to cell enlargement, which can be rescued by restoring cohesion.

This study reports the cryo-EM structure of the ZapA-FtsZ complex, revealing how ZapA interacts with FtsZ to regulate bacterial cell division.