Microtubule and mitosis dynamics

NDE1 Localizes to the Subdistal Appendages to Maintain Centrosome Integrity and Microtubule Organization

This study identifies NDE1's localization at subdistal appendages and shows its role in maintaining centrosome integrity and microtubule organization through interactions with other proteins.

Shepherding by biased diffusion organizes diffusible proteins on microtubules

The paper demonstrates that motors can shepherd diffusive proteins on microtubules through biased diffusion, revealing a mechanism for spatial patterning without direct binding.

Hierarchy of spindle forces in prometaphase

The paper analyzes 3D trajectories of chromosomes and spindle poles to reconstruct spindle forces in prometaphase, finding that pole-pole segregation is robust and primarily driven by interpolar forces.

Geometry shapes cytoplasmic Cdk1 waves that drive cortical dynamics

The paper investigates how cell geometry and nuclear Cyclin B-Cdk1 concentration influence cytoplasmic Cdk1 wave propagation and cortical dynamics in large embryos.

Yra2 regulates proteolysis of Cse4 to prevent its mislocalization to non-centromeric regions for chromosomal stability in budding yeast

This paper identifies Yra2's role in ubiquitin-mediated proteolysis of Cse4 to prevent its mislocalization and maintain chromosomal stability in budding yeast.

Eg5 activity and density-driven bundling organize the human metaphase mitotic spindle independently of spindle bipolarity

The paper shows that Eg5 organizes antiparallel microtubule bundles in human metaphase spindles through density-driven steric interactions, independent of spindle bipolarity.

Kinase interplay centered on two regulatory elements compartmentalizes the checkpoint protein Lte1 in S. cerevisiae

The paper identifies two regulatory elements in Lte1 that coordinate kinase activity to compartmentalize Lte1 at the bud cortex in S. cerevisiae, ensuring proper mitotic exit.

A myosin filament cage drives multiple aspects of mitosis unique to cells dividing within a polarized epithelium

The paper identifies a myosin filament cage in polarized epithelial cells that drives planar division and asymmetric cleavage, and shows its disruption leads to orthogonal division and multiple lumens.

Janusein and EB Cooperate to Enhance Microtubule Plus-end Catastrophe

This study identifies Janusein as a microtubule-associated protein that cooperates with EB proteins to enhance microtubule plus-end catastrophe.

Why is Cyclin A degraded before Cyclin B?

This study shows that rapid degradation of CyclinA ensures proper chromosome congression and genomic stability by preventing aneuploidy and malformed nuclei.

Divergence of Cortical Force-Generating Mechanisms Underlies Differences in Spindle Behavior between C. elegans and C. inopinata

This study compares spindle behavior in C. elegans and C. inopinata embryos, finding differences in pronuclear migration and spindle oscillations linked to changes in cortical force generation.

Drag racers and long haulers: the design space of myosin-V-like motor proteins

The paper models myosin-V-like motor proteins to show how they can achieve both high velocity and long run distances, identifying a trade-off and theoretical limits.

Microtubules sustain the fidelity of cellularization in a coenocytic relative of animals

This study investigates cellularization in Sphaeroforma arctica, showing microtubules help guide plasma membrane furrows to ensure equal partitioning of nuclei and cytoplasm during division.

Novel extracellular, extra-embryonic and intercellular γ-tubulin assemblies in mouse oocytes and preimplantation embryos

The paper identifies novel extracellular and intercellular γ-tubulin structures in mouse oocytes and embryos, revealing their role in early developmental processes.

The Endoplasmic Reticulum as a Cytoplasmic Anchor for Microtubule-Driven Aster Centration

The paper investigates how the endoplasmic reticulum (ER) contributes to microtubule aster centration in sea urchin zygotes through mechanical interactions and 3D modeling.

The KN domain of KANK proteins contains separable talin-binding and intramolecular interaction modules

This study identifies separable talin-binding and intramolecular interaction modules within the KN domain of KANK proteins, revealing a modular regulatory architecture.

Aurora kinase A enables collective invasion and metastasis by endowing a leader cell phenotype and stabilizing cohesion with follower cells

This study identifies Aurora kinase A's role in promoting collective cell invasion and metastasis by enabling leader cell phenotypes and stabilizing cohesion with follower cells.

Functional genetics reveals a role for the proteasome in regulating centromere assembly

The paper identifies the proteasome's role in regulating CENP-T levels to prevent non-centromere kinetochore assembly.

The Phosphorylation State of Melanophilin, a Myosin Va Adapter Protein, Biases the Tug-of-War Between Myosin Va and Kinesin-1 on Liposomes at Actin-Microtubule Intersections In Vitro

This study shows phosphorylated melanophilin biases liposome exit from actin-microtubule intersections toward actin, while dephosphorylated melanophilin biases exit toward microtubules.

Control of Golgi Architecture Through Three Centrosome Cohesion Pathways

This study identifies three centrosome cohesion pathways that regulate Golgi organization and function through distinct mechanisms.

Identification of Cysteine-Reactive Early Mitotic Inhibitors

This paper identifies novel early mitotic inhibitors using a cell-based high-content screening strategy with a cysteine-reactive compound library.

Pericentrin contains internal adaptor-like sequences that activate dynein directly

The paper shows that Pericentrin contains an internal sequence that directly activates dynein for co-translational transport, challenging the notion that dynein relies solely on N-terminal adaptors.

Mechanical characterization of human centromeres

This study uses optical tweezers to measure the mechanical properties of human centromeres, revealing stiffness at low forces and softening under tension, with implications for chromosome segregation.

Molecular mechanism of Aurora-A binding and activation by hnRNPK

This study identifies the molecular mechanism by which hnRNPK activates Aurora-A kinase through specific residue interactions in the Y-pocket.

Spindle size scaling secures mitotic fidelity against whole-genome doubling-associated mechanical stress

This study shows that whole-genome doubling increases mechanical stress on mitotic spindles, which is mitigated by spindle elongation to maintain mitotic fidelity.

Structural Basis of Human Kinetochore-Microtubule Coupling by the Ndc80 and Ska Complexes

Cryo-EM shows human Ndc80 and Ska complexes bound to adjacent microtubule protofilaments, linked by a SKA3 tether that passes Ska's preference for GTP-like lattice and tip tracking to Ndc80.

Force Induces a Collective Inactivity in Dyneins to Auto-Regulate Bidirectional Transport of Vesicles

The paper shows that dynein teams become collectively inactive under force, allowing kinesin to reverse cargo and regulate bidirectional transport.

Modeling centrosome positioning by actin--microtubule crosstalk and actin anchoring at the cell membrane

This study uses numerical simulations to show that ERM-mediated actin-membrane coupling is critical for centrosome centering and spindle geometry through interactions with microtubules and actin networks.

Organelle Volume Facilitates Spindle Positioning During C. elegans Female Meiosis

The paper shows that organelle volume in C. elegans oocytes influences spindle positioning during meiosis by affecting the central ball of organelles.

A mitotic restriction point for extrachromosomal DNA hubs in cancer cells

This study identifies mitosis as a critical window for ecDNA hub organization in cancer cells and shows WEE1 regulates hub integrity and oncogene expression.