Bacterial biofilms and quorum sensing

This paper develops a four-species polymicrobial biofilm model to study interspecies interactions in cystic fibrosis lung infections and observe clinically relevant phenotypes.

This study identifies PA3049, renamed BatR, as a key regulator of antibiotic tolerance in Pseudomonas aeruginosa biofilms, showing its role in stress response and antimicrobial resistance.

This study uses secondary metabolite profiling and whole-genome sequencing to identify rare genomic traits in Pseudomonas aeruginosa isolates that affect quorum sensing and metabolite biosynthesis.

This study shows that Staphylococcus aureus cells with lower buoyant density resist antibiotics more and form biofilms at air-liquid interfaces, while higher buoyant density cells are more antibiotic-sensitive and form biofilms on liquid-solid interfaces.

This study investigates how Pseudomonas aeruginosa adapts to malonate as a sole carbon source, revealing global gene expression changes and phenotypic responses.

This study examines how Pseudomonas aeruginosa adapts to repeated invasion of Staphylococcus epidermidis, showing divergent evolutionary responses between monoculture and coculture conditions.

This study investigates how sex hormones affect Pseudomonas aeruginosa iron acquisition and virulence in different media, finding hormone-specific changes in protein abundance and virulence pathways.

The paper proposes using lytic bacteriophages to reduce biofilm formation by fruit fly pathogens, potentially lowering disinfectant use and resistance pressure.

This paper investigates LuxR homologs in mycobacteria through computational analysis, revealing their domain diversity and potential roles in quorum sensing and other physiological processes.

This paper identifies BC1280 as an essential chaperone for biofilm-specific pili assembly in B. cereus, showing its role in filament formation and regulation of EPS expression.

This study identifies genes in Flavobacterium johnsoniae critical for surface colonization in both solitary and community settings using a model microbial community.

The paper describes a method to separate bacterial cultures into single cells and biofilm aggregates using mesh filters, validated by microscopy and DNA quantification.

The paper identifies highly specific LasR variants for HSL signals through mutagenesis and screening, improving signal discrimination in microbial consortia.

This paper shows H-NS inhibits swarming motility and promotes biofilm formation in Vibrio parahaemolyticus, with LeuO acting as an antagonist to H-NS.

This study evaluates cinnamic acid's anti-biofilm activity against oral bacteria and finds it reduces biofilm formation and biomass at higher concentrations.

This study shows that Candida albicans enhances Staphylococcus aureus virulence by inducing new, more aggressive colony variants through pH neutralization.

This study shows arginine reduces E. faecalis biofilm formation, alters gene expression, increases aggregation, and decreases susceptibility to antibiotics.

This study develops and benchmarks coculture microcolony experiments to quantify interspecific interactions and growth kinetics in bacterial communities.

This study shows that dispersin B and benzoyl peroxide synergistically reduce C. acnes biofilms by disrupting PNAG, leading to a >6 log reduction in CFUs.

The paper develops synthetic HSL sensors in Bacillus subtilis using LuxR-type regulators and combinatorial promoters, achieving up to 293-fold activation and identifying sequence-function relationships.

DeepGEEP predicts bacterial biofilm gene expression profiles using synthetic data and a 2D-CNN model, achieving higher accuracy than traditional machine learning methods.

This study compares two synthetic quorum sensing circuit architectures and evaluates three QS inhibition strategies to understand their steady-state behavior and parameter thresholds for effective suppression.

The paper models quorum sensing pathways with crosstalk and feedback, showing how crosstalk can enhance functional range and enable new activation modes.

The paper shows rhamnolipids from Pseudomonas aeruginosa colonies induce gel swelling over long distances, enabling collective motility and rescuing non-swarming mutants.

The paper demonstrates how microdroplet confinement can indirectly enrich a rare producer strain in a synthetic microbial community through spatial structure and metabolite diffusion.

This study uses mathematical modeling and experiments to show that growth rate fluctuations and bistability in a regulatory network explain single-cell heterogeneity in biofilm matrix production and sporulation in Bacillus subtilis.

The paper discovers that differentiating murine ES cells communicate over millimeters to form a macroscopic entity that survives if its population density exceeds a threshold.

The paper develops an inter-species quorum sensing communication system between cyanobacteria and heterotrophs to enable coordinated biological activities in mixed communities.

This study investigates how Pseudomonas aeruginosa phospholipase A (PlaF) affects biofilm formation and virulence by altering membrane phospholipid composition and regulating multiple pathways.