Cellular transport and secretion

The paper introduces an extended SIM-Pull protocol to study synaptic protein complexes and vesicle recruitment using TIRF microscopy.

The paper uses imaging to study calcium signals in zebrafish retinal bipolar cells, finding variations in local calcium levels related to ribbon size and active zone extent.

The paper presents secRecon, a comprehensive secretory pathway reconstruction, and uses it to identify secretory differences in plasma cells and CHO cells, highlighting potential targets for improving secretion capacity.

The paper uses proximity labeling to identify proteins interacting with 23 human RAB GTPases, revealing novel functional connections and interactions.

This paper reports the crystal structures of TMEM55B and its complex with a RILPL1 peptide, revealing a conserved TBM motif that mediates adaptor recruitment to lysosomes.

This study identifies TECPR2 as a Rab5 effector involved in endosomal cargo recycling, showing its role in integrin recycling and its loss-of-function effects in zebrafish.

The paper uses zap-and-freeze electron microscopy to study synaptic vesicle dynamics in mouse and human brain slices, revealing conserved endocytic mechanisms.

The paper shows IM30 binds membranes via a conserved helical hairpin, initiating assembly into higher-order structures that mediate membrane remodeling, revealing a conserved mechanism across ESCRT-III superfamily members.

This study uses CG-MD simulations to show that dynamin's GDP state induces conformational changes leading to membrane constriction.

The paper investigates the functional assembly of Qc-SNARE with Sec18 and Sec17 on membranes, revealing their roles in yeast vacuolar fusion and ATP hydrolysis.

The paper identifies two paralogues of NSF in Giardia, showing distinct localization and potential non-canonical functions in flagellar motility and stress adaptation.

This study investigates the subcellular distribution and interactions of late ESCRT paralogs in Giardia lamblia, revealing distinct functions and potential roles in encystation.

This study determines the molecular structure of the TPLATE complex and shows its structural flexibility is essential for membrane deformation during plant endocytosis.

This paper uses multiscale modeling to investigate how SNX1-SNX5 heterodimers recognize cargo and membranes in a retromer-independent manner.

The paper reveals how the endoplasmic reticulum (ER) is repurposed via autophagy to form nanopores in Drosophila olfactory organs, controlled by the Gox protein.

This study identifies a dependency on lysosomal exocytosis in FA pathway-deficient cells and tumors, revealing a new therapeutic target for FA-related cancers.

This study uses super-resolution microscopy to show that Rab6a regulates rhodopsin trafficking from the trans-Golgi in mammalian rod photoreceptors, with implications for retinal health.

The paper develops a nanobody that selectively blocks EFR3 recruitment of PI4KA by targeting TTC7B, reducing membrane recruitment and PI4P production.

The paper investigates the structural and mechanistic properties of the Ist2/Osh6 complex in lipid transport at membrane contact sites in yeast.

The paper shows electrostatic changes in the Exo70 subunit led to its dissociation from the exocyst complex, enabling functional divergence in plants.

This study shows poly-ubiquitylated transmembrane proteins outcompete mono-ubiquitylated and non-ubiquitylated proteins for endocytic uptake by clathrin-coated vesicles.

This study identifies STX1A as a lysosomal SNARE that regulates exocytosis by localizing to lysosomes and plasma membrane, affecting lysosome number, dispersion, and proteolytic activity.

This study identifies TMED proteins as key regulators of vesicle-dependent unconventional secretion, showing they mediate cargo selectivity through translocation and oligomerization.

This study identifies physical interactions between septins and the exocyst complex in fission yeast, showing septins guide exocyst localization for proper cytokinesis.

This study identifies Endophilin, Lamellipodin, and VASP as key components in fast endophilin-mediated endocytosis that regulate actin polymerization through liquid-like condensates.

This paper identifies RabD2 in E. histolytica, showing it self-associates via an EK-rich motif and regulates MVB biogenesis, leading to decreased parasite adherence through ubiquitination of GalNAc lectin.

This study shows that protein condensates and clathrin assembly regulate each other during clathrin-mediated endocytosis, enhancing vesicle formation while controlling condensate growth.

The paper shows that ErbB3 regulates Integrin β1 trafficking, redirecting it from recycling to lysosomal degradation and EV secretion when ErbB3 is lost, affecting cell migration.

This study identifies a mechanical regulation pathway involving Src-FAK-AMPK-GBF1 that controls cargo sorting and secretion from the Golgi in response to substrate stiffness.

This study determines the structure of the COPI vesicle coat with GOLPH3 and shows how it preferentially binds Golgi residents at late cisternae through coincidence detection of COPI and lipid.