Cellular transport and secretion

Clathrin-coated endocytic tubules mediate rapid and selective protein absorption by lysosome-rich enterocytes

This study identifies clathrin-coated endocytic tubules in lysosome-rich enterocytes that enable rapid and selective protein uptake in zebrafish and mice.

STRIPAK associates with Rabex5-Rabaptin5 to promote Rab5-dependent early endosome formation and EGFR transport

The paper identifies STRIPAK as a novel co-assembly factor of Rabex5-Rabaptin5, showing subunit-specific effects on early endosome formation and EGFR transport.

GolgiFold: A Structural Predictome of Human Golgi Protein Complexes

The paper develops GolgiFold, a structure-based PPI map of human Golgi proteins, identifying over 1,800 interactions and confirming new protein contacts through experimental validation.

Yeast PI(3,4,5)P3 controls vacuole fusion through Vam7 and membrane microdomain assembly

This study identifies PI(3,4,5)P3 as a regulator of yeast vacuole fusion, showing it controls vertex microdomain assembly and retains the SNARE Vam7 on membranes.

Evolutionary tuning of complexin-synaptotagmin fusion clamp governs regulated neurotransmitter release

The paper compares mammalian and C. elegans orthologs of Synaptotagmin-1 and Complexin in a single-vesicle assay to understand their roles in synaptic vesicle clamping and neurotransmitter release.

An iris-like pore for protein import into peroxisomes

The paper reports the structure of a peroxisomal import channel and shows how it adapts to transport proteins of different sizes.

TMEM55B promotes anterograde transport and distal axonal distribution of LAMP1-positive organelles

This study shows TMEM55B and TMEM55A regulate lysosome distribution in neurons by affecting motor protein transport, with TMEM55B specifically promoting anterograde transport of LAMP1-positive organelles.

Evolutionarily diverged on-switch for actin assembly in fungal endocytosis

The paper identifies Ldb17 as the on-switch for endocytic actin assembly in S. cerevisiae, revealing its complex regulation by Sla1 and evolutionary divergence from Dip1 in S. pombe.

Kv2-VAP interactions enhance presynaptic ER and mitochondrial calcium influx and the mobilization of vesicles from the reserve pool

This study shows that Kv2-VAP interactions at presynaptic membrane contact sites regulate Ca2+ signaling and synaptic vesicle mobilization from the reserve pool.

Multivalency in endocytic accessory proteins orchestrates membrane shape transitions

This study shows that multivalent SH3/PRM interactions between endophilin and lamellipodin regulate membrane curvature by suppressing tubulation through phase separation.

Quality control at the Golgi couples membrane proteostasis to lipid metabolism

The paper identifies a Golgi quality control checkpoint in yeast that links membrane proteostasis to lipid metabolism, showing that lipid imbalance from proteostasis defects is lethal.

Engineered proteins to restore and enhance neurotransmitter release

This paper engineers proteins to restore and enhance neurotransmitter release by reconstituting active zone functions in neurons.

The mannose 6-phosphate receptor system is ancient, widespread in eukaryotes, and functionally diversified in the human parasite Trichomonas vaginalis

The paper identifies the M6P pathway as ancient and widespread in eukaryotes, with functional diversification in Trichomonas vaginalis.

SNX13 and SNX14 interorganelle tethers suppress lysosome membrane damage and neurodegeneration

This study identifies SNX13 and SNX14 as proteins that maintain lysosome membrane integrity and prevent neurodegeneration by supporting lipid homeostasis and repair mechanisms.

Structure and function of the DFCP1 membrane barrier responsible for ER microdomain organization in lysosome repair

This study determines the structure of DFCP1 and shows it forms a barrier that concentrates PI3P for lysosomal repair.

The AP-1 clathrin adaptor has a conserved role in recycling resident proteins of the trans-Golgi network

This study investigates the role of AP-1 clathrin adaptor in recycling trans-Golgi network proteins, showing it maintains these proteins in the Golgi during maturation.

Heimdall SNAREs reveal an ancestral architecture of eukaryotic SNARE complexes

The paper characterizes SNARE-like proteins from Heimdallarchaeota, showing their four-helix bundle structure and evolutionary relationship to eukaryotic SNARE complexes.

Synaptotagmin-mediated plasma membrane homeostasis is required to recruit the endocytic machinery during cold stress

This study shows that SYT1 and SYT3 are essential for maintaining endocytosis during cold stress in Arabidopsis by preserving membrane composition and TPC subunit association.

ARF-1 Coordinates Cargo Sorting at FERARI Endosomal Recycling Hubs

This study identifies ARF-1 as a regulator of cargo sorting at FERARI-dependent endosomal recycling hubs through its cycling between active and inactive states.

Functional assignment of Golgi-associated vesicle tethers to specific membrane recycling pathways

The paper identifies specific Golgi-associated tethers and their roles in distinct membrane recycling pathways in yeast.

Dynamin-2 is a fission factor for SNX1-coated endosomal tubules

This study identifies Dynamin-2 as the fission factor for SNX1-coated endosomal tubules, showing it is required for tubule scission and cargo recycling.

Two Pleckstrin homology domains scaffold SNAP-47-containing SNARE complexes

The paper identifies and validates two PH domains in SNAP-47 and shows their role in stabilizing SNARE complexes for AMPA receptor insertion during synaptic plasticity.

A potent macrocyclic peptide binder enables effector-free crystallization of the GTPase RAB27A

The paper identifies a macrocyclic peptide binder, IMP2660, that disrupts RAB27A-effector interactions and enables crystallization of RAB27A without an effector, revealing its structural features.

Endosome-associated Rab GTPases control distinct aspects of neural circuit assembly

This study identifies the distinct roles of Rab5 and Rab11 in regulating neural circuit assembly through endosome-mediated trafficking.

A Tandem Fluorescent Rh1::eGFP mCherry Reporter Reveals Rhodopsin 1 Trafficking and Turnover In Vivo in Drosophila Photoreceptors

This study develops a fluorescent reporter to track Rhodopsin 1 trafficking and turnover in Drosophila photoreceptors, revealing acidified ER subdomains involved in Rh1 quality control.

Direct visualization of lysosome-mediated intracellular transport and membrane anchoring of TNF-α in human T cells

This study uses advanced imaging techniques to visualize and quantify TNF-α trafficking and membrane delivery in live human T cells, revealing mechanisms of intracellular transport and release.

Systematic Analysis of GARP- and EARP-Mediated Trafficking Pathways Using Lysosomal Proteomics

This study uses lysosomal proteomics and knockdowns to analyze the cargo spectra of GARP and EARP complexes, revealing their distinct roles in protein trafficking and their links to neurodegenerative disorders.

Rab35 drives epidermal wound closure by promoting lamellipodial protrusions and membrane vesicle transport

This study identifies Rab35 as essential for epidermal wound closure in Drosophila by promoting lamellipodia and membrane vesicle transport.

Dissipation of lysosome pH impairs formation and collapses existing LPS-induced lysosome tubules in macrophages

This study shows that lysosome pH is critical for LPS-induced lysosome tubulation in macrophages, as alkalinizing pH blocks tubulation while maintaining mTORC1 activity.

Survey of nucleotide-specific Rab GTPase interactions reveals multiple Rab effectors

The paper identifies nucleotide-specific interactions of Rab GTPases using yeast 2-hybrid and high-throughput sequencing, revealing new effector interactions and a novel Rab-binding domain.