Escherichia coli research studies

Regulatory plasticity of the rdar biofilm morphotype in clinical uropathogenic Escherichia coli and its modulation by ciprofloxacin

This study identifies rdar biofilm formation in clinical uropathogenic E. coli and shows subinhibitory ciprofloxacin suppresses it in resistant isolates.

Lipopolysaccharide and Wzi retain capsular polysaccharide at the Klebsiella pneumoniae cell surface and promote bloodstream infection fitness

This study identifies LPS and Wzi as factors retaining CPS on K. pneumoniae and shows their importance for bloodstream survival.

Host response and cell fates of human U937 monocytes upon infection with vancomycin-resistant E. faecium

This study examines how vancomycin-resistant E. faecium interacts with human U937 monocytes, showing early pro-inflammatory responses and isolate-specific cell death at later stages.

Amino Sugar Metabolism with AgaSY Coordinates Carbon Catabolism, Fimbrial Regulation, and Pathogenesis in Uropathogenic E. coli

This study identifies agaSY genes in UPEC as essential for amino sugar catabolism and links their loss to reduced virulence and colonization in a murine model.

Surface sensing and metabolic signals drive feedback regulation of long polar fimbriae in enterohemorrhagic Escherichia coli O157

This study shows that long polar fimbriae in E. coli O157 integrate metabolic and surface signals through regulatory feedback, with fimbrial structures promoting colonization without requiring tip adhesins.

Type 6 secretion systems of adherent-invasive Escherichia coli isolated from Crohn's disease patients shape intestinal microbiota.

This study shows that T6SS genes are more prevalent in Crohn's disease patients' microbiota and functionally impact gut microbiota composition in mice.

Repurposing clofarabine against enteric pathogens via metabolic starvation

This study identifies clofarabine as an inhibitor of EPEC pathogenesis by targeting bacterial and host translation, revealing a bacterial mechanism to counteract its effects.

Insights Into an Antibacterial Type VI Membrane-Disrupting Effector Of Adherent Invasive Escherichia coli

This study characterizes TmeAIEC, a T6SS effector from AIEC that disrupts bacterial membranes, and identifies its immunity protein TmiAIEC and key determinants for its activity.

Regulation of PL fimbriae (plf) and role of PlfB as a negative regulator during avian pathogenic Escherichia coli infection in turkeys

This study identifies PlfB as a negative regulator of PL fimbriae in avian pathogenic E. coli, showing its role in reducing fimbrial expression and bacterial colonization in turkey lungs.

A quantitative interactome map reveals affinity tailored hijacking of human cellular functions by resident and pathogenic bacteria

The paper reports a large-scale quantitative interactome of human PDZ domains and bacterial PDZ-binding motifs, showing bacteria tailor effector sequences to bind with similar or higher affinity than human PBMs to hijack host functions.

Targeting catecholate siderophore systems enables precision depletion of gut enterobacteria during colitis

This study shows that targeting catecholate siderophore systems reduces AIEC colonization in colitis by immunization, preserving gut microbiome composition.

Unveiling the small protein landscape of uropathogenic E. coli

This study identifies 338 previously unannotated small open reading frames in UPEC strain CFT073, with functional evidence for nine intergenic sORFs and potential roles in stress response.

Limited O-specific polysaccharide (OSP)-specific functional antibody responses in young children with Shigella infection in Bangladesh

This study examines antibody responses to Shigella in Bangladeshi children, finding that younger children have less effective functional antibody responses against OSP and proteins, correlating with disease severity.

Mapping the evolutionary path towards multi-drug resistance in the pandemic Escherichia coli ST131 lineage

This study traces the evolutionary path from ST131-B to ST131-C clades in E. coli by analyzing genomic data from ST131-B0 isolates, revealing genetic changes associated with antibiotic resistance and host adaptation.

The pod components of the Shigella T3SS sorting platform accommodate multiple copies of Spa33 (SctQ)

This paper models the Shigella T3SS pod complex using AlphaFold, showing two Spa33 proteins bind MxiK and SPOA2-SPOA2 domains, supporting a 1:4:2 stoichiometry and explaining electron density data.

Varied influence of curli on Shiga toxin-producing Escherichia coli O157, O26, O111 biofilm formation and animal cell adherence.

This study investigates the relationship between curli production, biofilm formation, and adherence of STEC isolates to bovine epithelial cells, finding that environmental conditions influence biofilm formation but not necessarily adherence.

Pathogenome and Plasmid-Borne Antimicrobial Resistance Phenotypes in a Multidrug-Resistant O111:H8 Shiga Toxin-Producing Escherichia coli Strain

The paper characterizes four plasmids in an O111:H8 STEC strain, showing their mobility, gene content, and resistance phenotypes, with findings on plasmid interactions and resistance mechanisms.

Cross-Serogroup Analysis of Representative Top-Seven Shiga toxin-producing Escherichia coli Plasmids Reveals Lineage-Specific Patterns

The paper analyzes plasmid sequences from seven STEC serogroups to identify lineage-specific virulence and resistance patterns and mobility features.

Shigella flexneri undergoes niche-specific adaptation during infection

This study identifies ZitB as a bacterial factor that enhances Shigella flexneri fitness during macrophage-associated metal stress by mitigating metal toxicity.

Mechanisms of mucosal immunity to oral Shigella infection in a physiological mouse model

This study establishes a mouse model to investigate adaptive immunity to oral Shigella infection and identifies CD4+ T cells as critical for cross-serotype protection through IFN-gamma and IgA production.

The small molecule regacin binds a conserved pocket in RegA to inhibit the expression of bacterial virulence factors

This paper identifies regacin as an inhibitor of RegA, a virulence regulator in Citrobacter rodentium, and defines its binding site through structural and mutagenesis studies.

Sequential invasion of epithelial and immune cells in Shigellosis

This study uses microscopy and transcriptomics to analyze the sequential invasion of Shigella flexneri in colonic tissue, identifying two infection stages and bacterial association with immune cells in the late stage.

Regulation and Characterization of the Cex system of enteric pathogens: a pathway for the lipidation and secretion of noncanoncial bacterial lipoproteins

This study characterizes the Cex system in enteric pathogens, showing that Rns regulates lipidation and secretion of noncanonical bacterial lipoproteins through a novel acyltransferase, CexD.

Global expression profile of Enterohemorrhagic Escherichia coli O157:H7 in phagosome of murine macrophages

This study profiles the global gene expression of EHEC O157:H7 in murine macrophage phagosomes under stress conditions, revealing survival strategies like SOS response activation and upregulation of stx2a.

Diverse horizontally transferred cellulose biosynthesis gene clusters in Escherichia coli strains

The paper identifies horizontally transferred cellulose biosynthesis gene clusters in Escherichia coli strains and their regulation by cyclic di-GMP.

Enterococcal Polysaccharide Antigen (EPA) rhamnan backbone contributes to cell wall architecture and is essential for antimicrobial resistance, innate immune evasion and phage infection

Genomovar-level resolution reveals rapid pathotype switching and genomovar-specific disease potential in diarrheagenic Escherichia coli populations in northern Ecuador

The paper analyzes whole-genome sequences of E. coli isolates to show rapid pathotype switching and genomovar-specific disease potential in northern Ecuador.

Effect of Temperature on Gene Expression of Escherichia marmotae

This study examines how temperature affects gene expression and protein abundance in Escherichia marmotae and E. coli, revealing distinct transcriptional and proteomic responses to 37°C versus 28°C.

Individual Host Variation in Single-cell Responses to Enteroaggregative Escherichia coli Infection Modeled with Human Colon Organoids

This study uses single-cell RNA sequencing on human colon organoids to show donor-specific responses to EAEC infection, revealing host-driven disease heterogeneity at the single-cell level.

Oxidative stress induces E. coli aryl polyene expression, sensitizing the bacterial stress response and modulating the redox environment of innate immune cells

This study shows that oxidative stress induces APE expression in E. coli, enhancing bacterial growth and modulating host immune cell redox environments.