Ubiquitin and proteasome pathways

The paper identifies ubiquitination as a key trigger for crinophagic degradation of secretory granules in Drosophila, mediated by Cnot4 and Usp7.

This study analyzes 11 USP7 variants in Hao-Fountain syndrome, showing they affect enzyme activity, stability, and substrate recognition, with some variants responsive to an allosteric activator.

This paper introduces UbyW, a platform for site-specific ubiquitylation using UBE2W, enabling efficient production of ubiquitylated proteins and identifying USP15 as a deubiquitylase for Ran K71 monoubiquitylation.

This paper shows PCMTD1, a cullin-RING ligase, recognizes L-isoaspartyl residues and targets damaged proteins for ubiquitylation and degradation.

The paper identifies the roles of Ubp2 and Ubp3 in editing polyubiquitin chains on ribosomes to maintain RQC activity.

The paper identifies a novel gel-like proteasome condensate, TAADs, that facilitates aggregate clearance but may contribute to neurodegenerative disorders if aggregates persist.

The paper identifies a mechanism where Deltex E3 ligases modify tankyrase with a ubiquitin-MAR hybrid mark, preventing PAR formation and stabilizing tankyrase through interaction with RNF114 and RNF166.

This study identifies DUB inhibitors that enhance bacterial clearance in Salmonella infections and shows their potential as host-directed therapies against antibiotic-resistant pathogens.

This study presents the crystal structure of SspH1 and analyzes its conformational dynamics, showing it exists in an open-closed equilibrium with substrate binding modulating state distribution but not inducing major conformational changes.

This study develops D-box peptides that inhibit APC/C by binding Cdc20, showing they are more potent than Apcin and can drive protein degradation.

The paper investigates how genotype-by-environment interactions influence the ubiquitin-proteasome system activity in yeast by analyzing genetic effects across different environments and substrates.

The paper identifies SUMO E1 covalent allosteric inhibitors that modulate polyamine synthesis through the MAT2A-AdoMetDC axis and show synergistic effects with polyamine inhibitors in T47D cells.

This study shows AMPK regulates proteasome phosphorylation and prevents persistent proteasome condensates in yeast by modulating kinase-phosphatase activity.

This study identifies the structural basis of VCP-VCPIP1-p47 complex formation and demonstrates the role of VCPID in enhancing DUB activity and Golgi reassembly.

The paper presents the cryo-EM structure of the UBR4 complex, revealing its architecture and mechanism for substrate binding and ubiquitin chain extension in protein quality control.

This study creates the first complete SUMO Cell Atlas in eukaryotes, revealing how SUMO components vary spatially and are regulated during stress responses in Arabidopsis roots.

The paper analyzes the expression and DNA methylation of 20S proteasome subunits across cancers, finding cancer-type dependent survival associations and functional pathway impacts.

This study identifies EIF3H as a regulator of ERK-driven oncogenic signaling in breast cancer metastasis, showing its role in promoting metastasis and sensitivity to chemotherapy.

The paper identifies a small molecule, BRD1732, that is ubiquitinated in cells, leading to proteasome inhibition through accumulation of ubiquitin chains.

This study identifies the Hb group's interaction with the hydrophobic pocket as sufficient to induce gate opening in archaeal 20S proteasomes, revealing a mechanism for proteasome activation.

The paper identifies USP15 as a fourth proteasome-associated DUB, showing it binds transiently to proteasomes and selectively disassembles short polyubiquitin chains.

This paper develops a recombinant Pf20S platform for structural analysis and inhibitor design, identifying a selective inhibitor with high potency against Plasmodium falciparum proteasome.

This study identifies SMURF2 and USP7 as regulators of KAP1 ubiquitination, showing that this dynamic cycle controls KAP1's SUMOylation activity and impacts chromatin structure and cancer progression.

The paper identifies the Cys-Arg/N-degron pathway as a major regulator of the cysteine-commencing proteome, revealing its role in hypoxia-induced metabolic adaptation and cell survival.

This paper develops two nanobody inhibitors of UCH37 to separately target its two Ub-processing activities, enabling their independent study.

The paper identifies HECTD4 as a tumor and metastasis suppressor that targets COX-2 for degradation, with COX-2 stabilization driving pro-tumorigenic effects in breast cancer.

The paper reports the cryo-EM structure of TXNL1-bound proteasome and identifies interactions critical for stress-induced degradation of TXNL1.

This study shows that proteasome activity inactivates ASK3 by degrading ASK1, linking proteasome function to cell survival under hyperosmotic stress.

This study shows that deleting Klhdc3 in mice causes obesity and developmental issues, highlighting its role in regulating protein stability and adiposity.

This study identifies the mechanism of cell cycle-dependent proteasome-mediated CdvB degradation in Sulfolobus acidocaldarius, revealing a role for a novel transcription factor and proteasome-activating nucleotidase.