Bacteriophages and microbial interactions

This paper uses Dynamic Light Scattering to assess phage bioactivity changes over time and optimize storage conditions for clinical phage applications.

This study identifies anti-defense proteins in phages that inhibit bacterial immune systems like Gabija, Thoeris, and Hachiman.

This study reveals the structural basis of the Gabija anti-phage defense complex and identifies a viral immune evasion mechanism through phage-encoded Gad1.

This study uses droplet microfluidics and fluorescence microscopy to analyze bacterial-phage interactions in E. coli, revealing growth and lysis dynamics of phage-infected cells.

The paper identifies and characterizes tailocins from Pantoea ananatis and Pantoea stewartii subsp. indologenes, showing they are derived from Myoviridae phages and exhibit killing activity against phytopathogens.

This study identifies XRE transcription factors in Caulobacter and its phage {varphi}CbK that regulate adhesin development and phage production through heteromeric interactions and gene repression.

The paper genetically engineers phages to produce Tailocins, which kill their native hosts, expanding antimicrobial options.

This study examines how extraction buffer chemistry affects extractable viral communities in different soil types, finding that spatial distance is the primary driver of community composition.

The paper identifies jumbo and non-jumbo phages effective against XDR Pseudomonas aeruginosa isolates causing eyedrop-associated infections.

The paper identifies 24 gene products from Mycobacteriophage Hammy that inhibit Mycobacterium smegmatis growth through a genome-wide overexpression screen.

The paper reports the isolation and genome sequencing of Pithoviridae, revealing genome organization, evolution, and the role of repeats in divergence from cedratviruses.

This study characterizes the phylogenetic and functional diversity of large and cell-associated viruses in two coastal sites of the Bay of Bengal, revealing high diversity and abundance of large phages and giant viruses.

This study shows that Vibrio cholerae reduces O1-antigen on its outer membrane in response to bile and anaerobic conditions, limiting phage adsorption by ICP1.

The paper examines interactions between lytic bacteriophages and Stevia rebaudiana extracts in bacterial environments, finding dynamic conditions alter extract effects based on phage and bacterial species.

This study shows that anti-Staphylococcus aureus phages are highly inhibited in plasma and synovial fluid due to clot formation, which prevents bacterial lysis.

The paper identifies novel lytic and lysogenic cyanophages that infect anatoxin-producing Microcoleus in benthic mats, influencing their population dynamics.

The paper develops a method to isolate and analyze protected environmental DNA (peDNA) from marine samples, revealing that extracellular vesicles are a major contributor to non-viral sequence space and play a significant role in horizontal gene transfer.

The paper describes a method for efficiently engineering phage genomes without using selection or CRISPR-Cas systems, achieving mutation rates of 1-14% for single-base substitutions, deletions, and insertions in three phages.

This study investigates the localization dynamics of two phage-encoded RNA polymerases during phiKZ infection, showing vRNAP is injected and excluded from the phage nucleus while nvRNAP is synthesized and localized within the nucleus.

This study shows that mucin promotes phage resistance in K. pneumoniae, but mucolytic agents like NAC reduce this resistance, suggesting their potential use in phage therapy.

This study identifies the O-antigenic polysaccharide as a receptor for phage {Phi}GP100 in Pseudomonas protegens CHA0, revealing its role in phage sensitivity and genomic plasticity of the OBC3 gene cluster.

The paper investigates how the choice of ultrafilter affects the recovery of bacteriophages from clinical samples, finding that pore size influences recovery strain- and sample-dependently.

geNomad identifies and annotates mobile genetic elements in sequencing data, discovering millions of new viruses and plasmids in public metagenomes.

The paper analyzes viral biogeography in wetlands, finding that dispersal, habitat filtering, and eco-evolutionary dynamics shape viral communities across space and time.

Phables improves phage genome recovery from fragmented viral metagenome assemblies, recovering more high-quality genomes and resolving variants with high nucleotide identity.

The paper determines the structure of the portal complex from Staphylococcus aureus Pathogenicity Island 1 transducing particles using cryo-electron microscopy, revealing its interaction with the capsid.

The paper characterizes novel recombinant mycobacteriophages generated through homologous recombination between two temperate phages, showing they are homoimmune to Island3 and can overcome antiphage defenses.

The paper identifies proteins interacting with the phage nucleus protein ChmA and describes their potential roles in genome packaging and transport.

This study isolates and characterizes three novel Crassvirales phages infecting Bacteroides cellulosilyticus WH2, revealing they do not co-evolve with their hosts and share a conserved tail spike protein involved in host interaction.

VIRify identifies and classifies viral contigs and prophages from metagenomic data with high accuracy across prokaryotic and eukaryotic viruses.