Insect Resistance and Genetics

This paper presents a high-quality genome assembly and CRISPR/Cas9 gene editing system for Tetranychus evansi, enabling studies of pesticide resistance and host adaptation.

This study identifies genetic mechanisms of pyrethroid resistance in La Reunion Island tiger mosquitoes, showing it results from local adaptation through detoxification gene over-expression and a large inverted superlocus.

This study identifies new candidate genes and tissues associated with resistance to a natural toxin in Drosophila species through genome-wide association and transcriptome analyses.

The paper presents a draft genome assembly of Leucoptera coffeella and identifies genes involved in dsRNA processing, supporting RNAi-based biopesticide development.

This study identifies CYP6Z genes in An. gambiae as drivers of broad-spectrum insecticide resistance through transcriptional upregulation and functional validation.

This study assesses pesticide resistance in Tetranychus urticae and Bemisia tabaci in Greece using bioassays and ddPCR, revealing widespread resistance mutations and highlighting the need for integrated pest management.

This paper presents a chromosome-level genome and genomic dataset for the invasive mite Halotydeus destructor, identifies pesticide resistance mutations, and develops KASP markers for resistance diagnostics.

The paper investigates the evolutionary origins of insecticidal protein families, finding evidence of divergent evolution and horizontal gene transfer across bacteria, ferns, and other organisms.

This study identifies and validates GmCad1 as a Cry1A receptor in Grapholita molesta, showing its binding to Cry1Aa, Cry1Ab, and Cry1Ac proteins.

The paper uses bulk segregant analysis to identify genomic regions linked to imidacloprid resistance in Colorado potato beetles and finds that RNAi reduces resistance, challenging its use for validation.

This study investigates whether sublethal exposure to Cry1 proteins in Spodoptera exigua larvae increases tolerance, finding no significant development of tolerance in larvae or their offspring.

This paper evaluates MgAl-LDH nanoparticles as a dsRNA delivery system for targeting jhp and chi genes in Phthorimaea absoluta, showing sublethal effects and gene expression reduction.

This study identifies Ugt35B1 as the key enzyme for nicotine glycosylation in Drosophila, revealing an insect detoxification pathway.

The paper identifies a superclone driving insecticide resistance in Acyrthosiphon kondoi across its invasive range through genomic and functional analyses.

This study identifies CHS2 gene knockout as a mechanism conferring high resistance to Vip3Aa in Spodoptera exigua and Agrotis ipsilon.

This study shows that dsDNA enhances dsRNA stability in Halyomorpha halys saliva, improving RNAi-mediated gene silencing but not significantly increasing mortality when delivered orally.

This study develops transgenic cowpea expressing synthetic BtCry1Ab, showing high resistance to legume pod borer and other lepidopteran pests without affecting growth or producing new metabolites.

This paper investigates the insecticidal potential of short and long antisense DNA oligos against Coccus hesperidum, finding that short 11-mer oligos achieve higher mortality rates than longer sequences.

The paper identifies and characterizes 22 cys-loop ligand-gated ion channel subunit genes in the Colorado potato beetle, including novel duplications and RNA editing events.

The paper identifies over-expression and copy number variation of cytochrome P450 and UDP-glucuronosyltransferase genes in ML-resistant Psoroptes ovis mites from cattle.

This study uses CRISPR/Cas9 to knockout Laccase2 in pea aphids, revealing its essential role in eggshell pigmentation, hardening, and survival during overwintering diapause.

The paper assesses insecticide resistance in West Nile virus vectors in Illinois, finding variation in resistance mechanisms across species and regions.

This study examines the microevolutionary link between digestion and insecticide resistance in tea tortrix moths, finding constitutively higher digestive gene expression in resistant larvae.

The paper identifies five kdr mutations in Indian Ae. aegypti populations and finds no recombination among haplotypes with V1016G, F1534C, and F1534L alleles.

This study identifies a novel gene, HaVipR1, responsible for Vip3Aa resistance in Helicoverpa armigera through genetic crosses and genomic analysis, showing its role in conferring resistance independently of known genes.

This study identifies and functionally characterizes an antenna-specific odorant binding protein in the Colorado potato beetle linked to insecticide resistance and host location.

This study identifies sequence features for effective dsRNA in insect pest control and develops a web-platform to optimize dsRNA design for RNAi.

This study shows chronic Bt spore ingestion in Drosophila reduces lifespan, causes gut inflammation, and promotes aging.

This paper investigates dsProsβ1-mediated proteasome targeting in the cabbage stem flea beetle using RNA degradomics and proteomics to reveal molecular mechanisms of RNAi-based pest control.