Mycobacterium research and diagnosis

Application of the ORBIT Protocol for Targeted Gene Deletion to Mycobacterium abscessus

This paper optimizes the ORBIT protocol for efficient gene deletion in Mycobacterium abscessus, enabling precise genetic manipulation for studying its physiology and pathogenesis.

Does Infection Progression of Mycobacterium abscessus Depend on Sex, Age, and Mouse strain?

This study evaluates how sex, age, and mouse strain affect M. abscessus infection progression using a beads model, finding BALB/cJRJ mice show more sustained infection with less variability.

Modelling the formation of skin ulcers observed in Buruli ulcer disease

This paper develops a mathematical model to study the dynamics of Buruli ulcer formation, showing that mycolactone concentration and bacterial diffusion rates influence wound expansion speed.

Carbon starvation of Mycobacterium abscessus induces a non-replicating state with extensive proteomic remodeling

This study identifies extensive proteomic changes in Mycobacterium abscessus under carbon starvation, highlighting the role of the DosRS system in non-replicating persistence.

Targeting homologous recombination repair to potentiate fluoroquinolone efficacy in Mycobacterium abscessus

This study identifies homologous recombination as a key mechanism of fluoroquinolone resistance in Mycobacterium abscessus and shows that targeting HR enhances FQ efficacy in vitro and in vivo.

Clinical isolates of Mycobacterium avium complex reveal an M. intracellulare-associated IL-17/neutrophilic pulmonary immune program in a murine disease model

The paper investigates immune responses to different Mycobacterium avium complex isolates in mice, finding that M. intracellulare induces a distinct IL-17/neutrophilic immune program compared to M. avium.

Reduced recombination relative to mutation characterizes dominant circulating clones of Mycobacterium abscessus

The paper analyzes genomic diversity in Mycobacterium abscessus, finding that dominant clones show increased accessory-gene gains and reduced recombination relative to mutation after establishment.

Tobramycin enhances Mycobacterium abscessus fitness through whiB7 induction

This paper shows tobramycin increases Mycobacterium abscessus survival by inducing whiB7, which enhances resistance to oxidative stress and macrophage killing.

Identification and characterization of ethR and ethA genes impacting the sensitivity of Mycobacterium abscessus to ethionamide

This study identifies EthR and EthA genes in Mycobacterium abscessus that mediate resistance to ethionamide, showing EthR is a more dominant resistance factor than NudC.

Parallel Emergence and Adaptive Evolution of Multicountry Circulating Mycobacterium abscessus Clones

The paper analyzes global M. abscessus genomes to show that recently emerged clones spread across countries and show shifts in selective pressures during expansion.

Expanding the scope of Mycobacterium abscessus reference strains to improve pulmonary disease modeling

This study identifies Mab clinical isolates that better model human pulmonary disease compared to the commonly used ATCC 19977 strain in mouse models.

Selected clinical strains of Mycobacterium avium unlock in vivo models for NTM drug discovery programs

The paper establishes a clinically relevant M. avium model using clinical isolates and longitudinal imaging to evaluate anti-mycobacterial drugs in vivo.

Emergence of plasmid-borne erm(55)-associated macrolide resistance in Mycobacterium chelonae and other rapidly-growing non-tuberculous mycobacteria in Europe

The paper identifies plasmid-borne erm(55) macrolide resistance in M. chelonae and other RGM in Europe, showing inducible resistance and genetic similarity to known plasmids.

NTM-DB: A Comprehensive Non-Tuberculosis Mycobacteria Genomic Database

NTM-DB provides a comprehensive genomic database for non-tuberculosis mycobacteria, offering genome assemblies, strain data, and analysis tools to support research and clinical applications.

The stress response factor SigH mediates intrinsic resistance to multiple antibiotics in Mycobacterium abscessus

This study identifies SigH as a key regulator of intrinsic antibiotic resistance in Mycobacterium abscessus through transcriptomic analysis and genetic complementation.

Transposon insertion sequencing identifies genetic determinants of intrinsic rifamycin resistance in Mycobacterium abscessus

This study uses transposon insertion sequencing to identify genes contributing to intrinsic rifamycin resistance in Mycobacterium abscessus, revealing new mechanisms and potential therapeutic strategies.

Analysis of publicly available genomic data

This study identifies genomic mutations associated with ethambutol resistance in Mycobacterium avium-intracellulare using clinical isolates and publicly available genomic data.

Diagnostic Value of Antibody Responses to Mycobacterium avium subsp. paratuberculosis-Derived Proteins PtpA and PtpB in Rheumatoid Arthritis

This study assesses anti-PtpA and anti-PtpB antibody levels as biomarkers for rheumatoid arthritis, finding higher levels in RA patients and improved diagnostic accuracy with a combined model.

First characterization of Mycobacterium avium subsp. paratuberculosis strain types in cattle from Kushiro Subprefecture, eastern Hokkaido, Japan

The study characterizes Mycobacterium avium subsp. paratuberculosis strain types in cattle from Japan, finding no Type S strains and suggesting prevalence of Type C strains.

A Murine Model of Mycobacterium abscessus Encapsulated in Alginate-Beads: Advancing Toward a Chronic Infection Model

This paper develops a murine model of Mycobacterium abscessus infection using alginate-encapsulated bacteria, showing slower clearance and increased dissemination, and evaluates antibiotic efficacy against encapsulated versus free bacteria.

Chemoproteomic elucidation of β-lactam drug targets in Mycobacterium abscessus

This study identifies β-lactam targets in Mycobacterium abscessus using chemoproteomics, revealing 17 conserved targets and demonstrating imipenem's superior inhibition over cefoxitin.

Sulbactam-Durlobactam Plus Ceftriaxone Dosing and Novel Treatment Regimens for Mycobacterium abscessus Lung Disease

This study evaluates sulbactam-durlobactam plus ceftriaxone dosing and combination regimens for Mycobacterium abscessus lung disease, finding that sulbactam-durlobactam-ceftriaxone achieves the highest microbial kill in a hollow fiber model.

Antibiotic dose-response curves can measure antibiotic activity against Mycobacterium abscessus and Mycobacterium peregrinum

This study uses dose-response curves to measure antibiotic activity against Mycobacterium abscessus and Mycobacterium peregrinum, showing that IC50 and IC75 are more consistent than MIC.

Novel regimens for treatment of Mycobacterium avium lung disease based on advanced in vitro systems and the mathematics of basis functions

The paper evaluates new drug regimens for Mycobacterium avium lung disease using an in vitro model and basis functions, finding that ceftriaxone-omadacycline-rifabutin outperforms the standard-of-care.

Strain-dependent disease progression and necrotizing granuloma formation induced by virulent Mycobacterium avium complex strains in a murine model

This study evaluates strain-dependent disease progression and necrotizing granuloma formation in a murine model of MAC-PD using five clinical MAC strains.

Mutations in the ubiA gene are the major mechanism of ethambutol resistance in Mycobacterium avium

This study identifies ubiA gene mutations as the primary mechanism of ethambutol resistance in M. avium by analyzing clinical strains and resistant mutants.

Ribosomal protection as a linezolid resistance mechanism in Mycobacterium abscessus

This study identifies an ABC-F protein in Mycobacterium abscessus that confers resistance to linezolid and other ribosome-targeting antibiotics.

Genetic analysis of Mycobacterium abscessus reveals genomic diversity linked to global plasmid distribution

The paper analyzes plasmid distribution in Mycobacterium abscessus using whole genome sequencing to show how plasmid transfer increases genomic diversity and aids in estimating bacterial contact history.

Alpha-1-antitrypsin (AAT) inhibits Mycobacterium intracellulareinduction of monocyte colony stimulating factor: another host defense function of AAT

This study shows AAT inhibits MAC-induced M-CSF but not GM-CSF in macrophages, suggesting a role in modulating host defense against mycobacteria.

A division-associated envelope protein, MAB_2363, drives intrinsic resistance and virulence in Mycobacterium abscessus

This study identifies MAB_2363 as a division-associated protein that contributes to intrinsic resistance and virulence in Mycobacterium abscessus by affecting cell division and antibiotic susceptibility.