Biosensors and Analytical Detection

This paper develops CRISPR-based assays for rapid and accurate detection of DNA and RNA shrimp viruses, showing comparable sensitivity and specificity to real-time PCR.

The paper develops a low-cost, smartphone-operated PCR thermal cycler for detecting antimicrobial resistance genes in human urine samples, showing good thermal stability and accuracy.

The paper develops hierarchical Bayesian models to analyze LAMP amplification data and identifies factors affecting assay resolution using clinical samples and synthetic data.

The paper introduces a PAM-less Cas12a-based assay for rapid, sensitive visual detection of Vibrio species without requiring a PAM site, using T7 exonuclease and low-cost materials.

The paper develops a triplex lateral flow assay for detecting Nipah virus, MERS-CoV, and REBOV genomes with high sensitivity and specificity.

This paper develops a rapid, sensitive, and point-of-care FV3 detection system combining RPA-CRISPR/Cas12a with smartphone microscopy and deep learning, achieving 100% accuracy in classification.

The paper develops an electricity-free nucleic acid extraction method from dried blood spots on filter paper for point-of-care diagnostics, showing comparable performance to standard methods.

This study compares AS-Mini-LAMP and PSR for amplifying short DNA templates, finding AS-Mini-LAMP more effective for mutant allele detection.

This paper develops a paper-based isothermal amplification method for rapid nucleic acid quantification using fluorescent nucleation site analysis, achieving results in under 20 minutes.

This paper develops a CRISPR/Cas12a-based colorimetric assay with a convolutional neural network for rapid, naked-eye detection of viral nucleic acids in point-of-care testing.

This paper demonstrates the use of lysate-based cell-free biosensors integrated with personal glucose monitors for on-site analyte quantification.

This paper introduces a protocell array platform for simultaneous detection of diverse analytes, demonstrating its ability to measure small molecules, minerals, and nucleic acids from a single sample.

This study attempts to develop an aptamer lateral-flow assay (ALFA) for detecting α-amanitin but fails to produce a colorimetric readout due to challenges with small molecule immobilization and binding mechanisms.

This study develops a direct multiplex RPA method for rapid, sensitive detection of S. aureus and P. aeruginosa in food samples without DNA extraction.

This paper introduces CECRID, a CRISPR-based detection system enhanced with L-proline to improve sensitivity and robustness for nucleic acid detection, particularly for SARS-CoV-2.

This study develops a rapid, sensitive RPA method for detecting L. monocytogenes in crude samples, achieving 1000-fold higher sensitivity than conventional PCR.

This study develops and validates a colorimetric LAMP assay for rapid, in-field detection of African swine fever virus using crude serum samples during an outbreak in Vietnam.

This paper develops a low-cost smart system for DNA/RNA detection using visible light and IoT techniques, enabling qualitative and semi-quantitative analysis without UV equipment.

Rapidemic is a label-free DNAzyme-based platform for nucleic acid detection using RPA and LSDA, demonstrating visible color change without complex instrumentation.

This paper introduces a yeast biosensor that detects pharmaceuticals via scent, requiring no equipment, and demonstrates its sensitivity to estradiol in low-resource settings.

The paper introduces RPA-PCR couple to bypass DNA extraction in plant diagnostics, achieving specific detection of Phytophthora pathogens with sensitivity down to 10 fg of genomic DNA.

This paper develops CRISPR-Cas13a-based diagnostics for real-time detection of Ebola and Lassa viruses in field settings, demonstrating sensitivity and usability in clinical samples.

This paper introduces Apta-NASBA, a method for highly specific, multiplexed pathogen detection using fluorescent aptamers, achieving a 1 pM detection limit and compatible with various detection platforms.

This paper develops a low-cost food pathogen diagnostic workflow for low-resource settings and demonstrates its effectiveness in detecting E. coli O157 contamination in Cambodian produce.

This paper develops a CRISPR/Cas12a-based method for detecting Mycobacterium tuberculosis with high sensitivity and specificity using RPA and fluorescence detection.

This paper develops a rapid, sensitive, and on-site ASFV detection system using RAA-Cas12a and lateral-flow strips, achieving femtomolar sensitivity in an hour.

This paper presents a low-cost, portable PCR platform using a graphene-based chip and disposable thermocycler for nucleic acid amplification, enabling visual monitoring and point-of-care testing.

This paper develops a rapid, sensitive, and specific MCDA-LFB assay for detecting the mcr-1 gene in clinical and livestock samples.

This paper develops a portable, low-cost diagnostic system for detecting plant pathogens using synthetic biology and isothermal amplification with colorimetric output.

This paper develops a multiplex isothermal diagnostic assay for detecting variable viral targets, demonstrating its effectiveness against divergent Zika virus lineages.