Biosensors and Analytical Detection

This paper introduces a portable microfluidic device for highly multiplexed nucleic acid detection, demonstrating its integration with CRISPR-based technology for rapid pathogen and variant detection.

The paper demonstrates the use of a wearable fluorescence imaging system to detect porphyrin-specific fluorescence in pathogenic bacteria, showing its effectiveness in identifying bacterial strains associated with infections.

This paper develops a CRISPR-based assay using Cas12a orthologs for detecting NDRV S3 gene with high sensitivity and visual detection capability.

The paper evaluates a multiplex LAMP assay for detecting STIs and fluoroquinolone resistance, showing high specificity and sensitivity.

The paper develops a field-deployable water bath, IsoHeat, for LAMP reactions, which maintains target temperatures and operates efficiently in various environmental conditions.

qByte is an open-source, affordable fluorimeter that performs reliable biological tests in various settings, validated through real-world applications.

This paper introduces a CRISPR-on-beads assay for rapid, sensitive, and specific detection of MRSA bacterial DNA using isothermal amplification, CRISPR cleavage, and magnetic bead signal amplification.

This paper develops a paper-based LAMP assay for detecting H5 subtype avian influenza virus with a detection limit of 500 copies per reaction, offering a low-cost, user-friendly diagnostic tool for resource-limited settings.

The paper introduces MFAST, a diagnostic chip for rapid, sensitive, and specific detection of Leishmania species, enabling duplex detection and stable performance in low-resource settings.

This paper develops a paper fluidic device for continuous cocaine monitoring in simulated sweat over 17 hours using an aptamer-based biosensor and evaporation-driven fluidics.

This paper develops a multiplexed RT-LAMP test for detecting SARS-CoV-2, Influenza, and RSV in saliva with high accuracy using a machine-learning-enabled portable analyzer.

This paper introduces a modular cell-free biosensing platform called TLISA for rapid, equipment-free protein detection using split T7 RNA polymerase and affinity domains.

This paper develops a rapid, inexpensive, and sensitive dot-blot assay using chitosan gold nanoparticles to detect histidine-tagged recombinant proteins with a low limit of detection.

The paper develops a colorimetric CRISPR biosensor for detecting Salmonella Typhi DNA, using β-galactosidase to produce a visible blue color without requiring specialized equipment.

This paper introduces a low-cost hybrid system for rapid blood plasma separation, achieving high efficiency and accuracy for on-site diagnostics.

The paper introduces the CandyCollect O2C microfluidic system to automate antigen elution for rapid detection of group A streptococcus, showing comparable or better results than manual methods.

The paper develops a cholera RDT that detects both Vibrio cholerae and the bacteriophage ICP1, demonstrating its ability to detect ICP1 as a proxy for pathogen presence.

DIYNAFLUOR is a low-cost, open-source nucleic acid fluorometer designed for eDNA quantification in resource-limited settings, offering an affordable alternative to commercial fluorometers.

The paper develops and optimizes a one-pot NASBA-Cas13a assay for rapid, sensitive detection of SARS-CoV-2 RNA without specialized equipment.

The paper introduces a dual probe-based isothermal amplification method for mutation detection, achieving high accuracy in identifying drug resistance mutations in Mycobacterium tuberculosis.

This paper develops high-throughput methods to optimize cell-free biosensors on paper, achieving cost-effective and heat-tolerant formulations for field use.

This paper enhances lateral flow assay sensitivity by using laser-machined microchannels in nitrocellulose membranes to delay flow and increase immunological reaction time.

This paper develops an enzyme-free nucleic acid test using hybridization chain reaction and paper-based filtration to detect sub pM DNA levels, mimicking in cellulo conditions for point-of-care diagnostics.

This paper introduces a rapid, sensitive, and visual detection method for canine parvovirus using endonuclease activities and isothermal amplification.

The paper develops a lateral flow assay using chimeric antigens to detect West Nile virus seroconversion in crocodiles, achieving 100% sensitivity and specificity.

The paper develops an algorithm for designing LAMP assays and a computer vision tool for analyzing colorimetric outputs to detect antimicrobial resistance in Vibrio cholerae.

This paper develops a step-emulsion microfluidic device combined with digital-RPA for rapid, sensitive, and quantitative detection of Vibrio parahaemolyticus with a detection limit of 10 CFU/L.

The paper introduces a portable fluorescence viewer for rapid molecular assay readout in space and low-resource settings, demonstrating its use for SARS-CoV-2 detection and gene expression analysis.

WELPCR is a low-cost, portable PCR device designed for nucleic acid amplification and sensing, offering an affordable solution for educational and research labs.

This paper develops a CRISPR-based diagnostic method using CO1 gene targeting to detect invasive moth pests with high accuracy and sensitivity.