Zebrafish Biomedical Research Applications

This study shows that early-life stress in zebrafish affects anxiety-related behaviors and these effects are transgenerational across three generations.

This study shows that arnt1 and arnt2 genes are required alongside npas4l for hemato-vascular specification in zebrafish embryos, with each gene necessary but not sufficient alone.

This study examines the effects of quinpirole, a dopamine D2/D3 receptor agonist, on behavior and neurotransmitter levels in adult zebrafish, finding altered locomotion, anxiety, memory, and neurotransmitter profiles.

The paper introduces an efficient CRISPR-Cas9-based knock-in method in zebrafish for lineage tracing and generates reporter lines for specific cell types.

The paper describes how ZFIN annotates phenotype, expression, and disease model data in zebrafish research across different experimental designs.

The paper identifies three distinct neural pathways in larval zebrafish that mediate habituation to dark looming stimuli, with one pathway specifically inhibiting escape responses.

This study evaluates a semi-automated tool to quantify craniofacial variations in zebrafish models of human skeletal diseases, finding phenotypic differences for four genes and parallels to human symptoms for two.

The paper shows that actomyosin contractility in olfactory placode neurons causes the skin epithelium to rupture, forming the nostril in zebrafish.

The paper generates a transparent killifish line by inactivating three pigmentation genes, enabling in vivo studies of aging and behavior.

The paper creates a single-cell gene expression atlas of the larval zebrafish brain using HCR, identifies a novel cerebellar cell type, and integrates gene expression with functional activity maps.

This study identifies Duox as a key NADPH oxidase for ROS production and fin regeneration in adult zebrafish, with secondary roles for Nox1-4 and a circadian rhythm in ROS levels.

This study identifies Zbtb14 as a regulator of monocyte and macrophage development by inhibiting PU.1 expression in zebrafish, with implications for myeloid disorders.

The paper introduces DAMAKER, a 3D tool for tracking tissue growth and neuronal differentiation in zebrafish hindbrain over time using in vivo imaging and segmentation.

This study identifies a cerebellar-prepontine circuit that mediates tonic immobility in larval zebrafish in response to inescapable threats.

This study shows that Pcdh18a in stromal cell progenitors is essential for hematopoietic niche formation in zebrafish.

This study shows that zebrafish with nonfunctional lateral lines have reduced pth2 expression in social contexts, suggesting they experience isolation despite being raised socially.

This paper demonstrates that two- and three-photon microscopy can access the entire adult Danionella brain without modifications, enabling whole-brain imaging in live adult vertebrates.

The paper presents a collection of zebrafish genetic mutants for studying mitochondrial biology and disease.

The paper identifies a tecto-thalamic neural circuit that processes visual social signals and drives social attraction in zebrafish.

This study investigates innate directional and color preferences in male and female zebrafish using maze tasks, finding sex differences in color preference but not direction.

The paper develops viral tools for conditional and transneuronal gene expression in zebrafish to study neuronal circuits.

The paper shows macrophages help regenerate neuromasts in zebrafish by disrupting Schwann cell inhibition of interneuromast cells during injury.

This study shows that osteoblast dedifferentiation and migration are distinct, differentially regulated processes required for zebrafish fin regeneration.

The paper develops a CRISPR/Cas9-based method to generate Cre/lox regulated conditional gene alleles in zebrafish, enabling cell-type specific gene inactivation.

The paper maps the development of cell populations in the zebrafish telencephalon during eversion using transgene expression and creates a structural atlas.

This study uses computational modeling to show that growth orientation, not growth rate, primarily drives jaw joint morphogenesis in larval zebrafish.

This study shows that ywhaz gene deficiency in zebrafish affects brain activity and behavior, with altered neuronal connectivity and monoamine levels.

The paper identifies three genes associated with heart failure using human exome sequencing and validates their roles in cardiovascular function using zebrafish CRISPR/Cas9 mutagenesis.

This study uses light-sheet microscopy to investigate spontaneous activity in the developing zebrafish lateral-line system, showing that hair cells and surrounding cells autonomously generate spontaneous calcium activity.

This study examines the role of L-plastin in macrophage migration and morphology in zebrafish, finding that its absence impairs migration speed and directionality.