Zebrafish Biomedical Research Applications

The paper uses live-cell imaging to study neural network development in zebrafish, revealing conserved structural dynamics and optimization strategies.

This study uses fluorescent protein knock-in lines in zebrafish to track Collagen4 remodeling in cardiovascular development and regeneration.

Zebrafish larva infection experiments plus pharmacokinetic-pharmacodynamic (PKPD) modelling gave bedaquiline's in vivo Emax and EC50 against M. marinum in about 2 months; waterborne clarithromycin barely entered larvae.

The paper develops a multiplex CRISPR-Cas9 gRNA toolkit to generate fully unpigmented zebrafish larvae with minimal mortality, enabling improved imaging without chemical inhibitors.

This study quantifies spatial asymmetry in zebrafish pectoral fin regeneration, revealing differential gene expression and signaling dynamics between anterior and posterior regions.

The paper introduces an open-source VR platform for head-fixed adult zebrafish to study behavior and neuronal activity in controlled virtual environments.

gene2fish arranges 53,595 Thisse in situ hybridization images of 8,263 zebrafish genes into a gene-by-stage grid, with anatomy-term search for validating scRNA-seq markers.

This study optimizes breeding conditions for Danionella cerebrum to enhance egg production and collection efficiency.

This study shows laminin and fibronectin guide endothelial self-organization during intersegmental vessel formation in zebrafish by constraining network-forming behavior.

This paper develops a motor endurance assay for zebrafish larvae to study neurological diseases, finding reduced endurance in TDP43-overexpressing larvae and MPP+-treated larvae.

The paper identifies cell types expressing Negr1 in zebrafish and links its expression to diverse traits, suggesting broad gene action.

This study defines zebrafish meningeal development, identifies conserved Foxc1 and Wnt signaling roles, and reveals limited regenerative capacity and developmental defects upon meningeal loss.

The paper creates a zebrafish hindbrain atlas linking functional ensembles, gene expression, and behavior through light-field recordings and molecular data.

This paper introduces a novel cranial window surgery technique for adult zebrafish that ensures high survival rates and preserves neurovascular integrity for in vivo manipulations.

This study identifies sexual dimorphism in zebrafish marrow that affects blood cell composition and immune response, with parallels to human hematopoiesis.

The paper creates a detailed neuroanatomical atlas of the adult Danionella telencephalon, revealing larval-like features and demonstrating their functional relevance.

The paper identifies V2a neurons in zebrafish as key mediators of long-range locomotor propagation through their connectivity patterns.

zAcademy is an open-source conditioning arena used to study avoidance learning in zebrafish, revealing sex- and genotype-dependent differences in learning and memory.

The paper identifies a moving front of osteoblast maturation that controls proliferation during zebrafish scale regeneration, explaining size scaling through regulated cell dynamics.

The paper investigates neural activity differences between bold and shy zebrafish during learning, finding distinct functional connectivity patterns in brain regions associated with reward processing.

This study examines the effects of unpredictable chronic stress on zebrafish, finding increased anxiety and decreased social preference, suggesting stress-induced neurobiological changes.

This study examines how personality type, sex, and food reward affect learning and memory retention in zebrafish using a conditioned place preference paradigm.

The paper identifies hoxb5a as a critical regulator of spinal cord regeneration in zebrafish by analyzing transcriptional and microenvironmental differences between successful and failed regeneration cases.

This study analyzes social behavior in young zebrafish, finding that they exhibit specific proximity preferences and turning biases that shape early social interactions.

In larval zebrafish, 68% of 81 traced serotonergic dorsal raphe neurons target only one of telencephalon and spinal cord; stress in week 1, not week 2, raised telencephalic fiber density.

This study evaluates the effects of electrical stunning on stress response and tissue integrity in larval zebrafish, finding no significant stress response and preserved brain tissue.

This study creates a zebrafish Runx1 lineage tracing tool to track hematopoietic development and adult blood cell lineages.

This study uses zebrafish embryos to screen chemicals for developmental toxicity and identifies mechanisms, showing calcium homeostasis is involved in purpurin toxicity.

Two-photon optical backfills of pan-neuronal photoactivatable GFP in larval zebrafish yield an estimated 1,300 spinal-projecting neurons per larva across hindbrain, midbrain and diencephalon, with tentative homologies to mammalian nuclei.

This paper develops a computational method to map full 3C-3D blood flow in the beating zebrafish heart using SPIM and microPIV techniques.