Zebrafish Biomedical Research Applications

Structural Evolution of Neural Networks during Zebrafish Early Development

The paper uses live-cell imaging to study neural network development in zebrafish, revealing conserved structural dynamics and optimization strategies.

sox17 mutagenesis reveals microglia can be impacted by genetic maternal effects

The paper identifies a genetic maternal effect on microglia development through sox17 mutation in zebrafish, showing that maternal genotype impacts microglia and yolk sac precursor cells.

Collagen4 fluorescent protein knock-in lines reveal remodeling of cardiovascular basement membranes

This study uses fluorescent protein knock-in lines in zebrafish to track Collagen4 remodeling in cardiovascular development and regeneration.

Accelerating drug development in infectious diseases using zebrafish disease models supported by pharmacokinetic pharmacodynamic modeling as new approach methodology (NAM)

Zebrafish larva infection experiments plus pharmacokinetic-pharmacodynamic (PKPD) modelling gave bedaquiline's in vivo Emax and EC50 against M. marinum in about 2 months; waterborne clarithromycin barely entered larvae.

I. Phantom: a multiplex CRISPR-Cas9 gRNA toolkit for efficient generation of fully unpigmented F0 zebrafish

The paper develops a multiplex CRISPR-Cas9 gRNA toolkit to generate fully unpigmented zebrafish larvae with minimal mortality, enabling improved imaging without chemical inhibitors.

Asymmetric regeneration dynamics in zebrafish pectoral fins

This study quantifies spatial asymmetry in zebrafish pectoral fin regeneration, revealing differential gene expression and signaling dynamics between anterior and posterior regions.

Open-source virtual reality platform for head-fixed adult zebrafish

The paper introduces an open-source VR platform for head-fixed adult zebrafish to study behavior and neuronal activity in controlled virtual environments.

gene2fish: a multi-gene spatial expression browser for in situ hybridization data across zebrafish development

gene2fish arranges 53,595 Thisse in situ hybridization images of 8,263 zebrafish genes into a gene-by-stage grid, with anatomy-term search for validating scRNA-seq markers.

Optimization of breeding conditions in Danionella cerebrum to advance its use as a vertebrate model

This study optimizes breeding conditions for Danionella cerebrum to enhance egg production and collection efficiency.

Laminin and Fibronectin contribute to Guided Endothelial Self-Organization during Intersegmental Vessel Formation

This study shows laminin and fibronectin guide endothelial self-organization during intersegmental vessel formation in zebrafish by constraining network-forming behavior.

A motor endurance assay for zebrafish larvae: proof of principle in chemical and genetic models of neurological disease

This paper develops a motor endurance assay for zebrafish larvae to study neurological diseases, finding reduced endurance in TDP43-overexpressing larvae and MPP+-treated larvae.

Cellular and molecular census of the pleiotropic disease locus Negr1 in zebrafish

The paper identifies cell types expressing Negr1 in zebrafish and links its expression to diverse traits, suggesting broad gene action.

Conserved cellular architecture and developmental mechanisms of the zebrafish meninges

This study defines zebrafish meningeal development, identifies conserved Foxc1 and Wnt signaling roles, and reveals limited regenerative capacity and developmental defects upon meningeal loss.

A Gata4-Loxl2 axis controls ECM remodeling to enable scar-free spinal cord regeneration in zebrafish

This study identifies the Gata4-Loxl2b axis as critical for scar-free spinal cord regeneration in zebrafish by regulating ECM remodeling and glial function.

An atlas of functional ensembles in the zebrafish hindbrain links structural and molecular features to behavior

The paper creates a zebrafish hindbrain atlas linking functional ensembles, gene expression, and behavior through light-field recordings and molecular data.

A Novel Single-Fish Assay for Ethanol Self-Administration in Zebrafish

This study introduces an individual self-administration assay in zebrafish to model ethanol abuse and study its behavioral and neurobiological effects.

A highly reproducible, safe cranial window surgery for targeted in vivo manipulations in the adult zebrafish telencephalon

This paper introduces a novel cranial window surgery technique for adult zebrafish that ensures high survival rates and preserves neurovascular integrity for in vivo manipulations.

Sexual dimorphism in adult zebrafish marrows regulates blood cell composition and immune response

This study identifies sexual dimorphism in zebrafish marrow that affects blood cell composition and immune response, with parallels to human hematopoiesis.

Atlas of adult Danionella fish telencephalon reveals retention of larval characters

The paper creates a detailed neuroanatomical atlas of the adult Danionella telencephalon, revealing larval-like features and demonstrating their functional relevance.

Spinal V2a neurons support locomotor propagation via long-range connectivity

The paper identifies V2a neurons in zebrafish as key mediators of long-range locomotor propagation through their connectivity patterns.

zAcademy: an open-source programmable conditioning arena reveals sex- and genotype-dependent avoidance learning in adult zebrafish

zAcademy is an open-source conditioning arena used to study avoidance learning in zebrafish, revealing sex- and genotype-dependent differences in learning and memory.

A moving front of osteoblast maturation scales regenerating zebrafish bone

The paper identifies a moving front of osteoblast maturation that controls proliferation during zebrafish scale regeneration, explaining size scaling through regulated cell dynamics.

Differences in interactions between brain regions across personality types while learning in zebrafish (Danio rerio)

The paper investigates neural activity differences between bold and shy zebrafish during learning, finding distinct functional connectivity patterns in brain regions associated with reward processing.

Unpredictable chronic stress produces a mixed depression/anxiety behavioral profile in zebrafish

This study examines the effects of unpredictable chronic stress on zebrafish, finding increased anxiety and decreased social preference, suggesting stress-induced neurobiological changes.

Personality Variation in Associative Learning and Memory Retention in Zebrafish (Danio rerio)

This study examines how personality type, sex, and food reward affect learning and memory retention in zebrafish using a conditioned place preference paradigm.

Microenvironmental and transcriptional determinants of the robustness of spinal cord regeneration in zebrafish

The paper identifies hoxb5a as a critical regulator of spinal cord regeneration in zebrafish by analyzing transcriptional and microenvironmental differences between successful and failed regeneration cases.

Social modulation of activity and orientation fosters complex interactions in young zebrafish

This study analyzes social behavior in young zebrafish, finding that they exhibit specific proximity preferences and turning biases that shape early social interactions.

Organization, development, and plasticity of the serotonergic dorsal raphe nucleus projectome

In larval zebrafish, 68% of 81 traced serotonergic dorsal raphe neurons target only one of telencephalon and spinal cord; stress in week 1, not week 2, raised telencephalic fiber density.

A transgenic zebrafish for spatiotemporal optogenetic activation of FGF/ERK signaling

The paper introduces a transgenic zebrafish line that enables precise optogenetic activation of FGF/ERK signaling in vivo.

An applicable and efficient retrograde monosynaptic circuit mapping tool for larval zebrafish

This paper develops an efficient method for retrograde monosynaptic tracing in larval zebrafish neural circuits using modified rabies viruses.