Advanced NMR Techniques and Applications

This paper introduces BPHON, a method that uses image analysis to score the fitness of protein structures against NMR spectra without requiring full resonance assignments.

This paper introduces ANSA, an automated NMR spectral alignment tool using cryo-EM motion correction, improving alignment accuracy and reducing manual effort in NMR data processing.

This paper introduces a 2D NMR technique for homonuclear chemical shift correlation in solids under MAS, showing rapid spin-exchange between nearby 15N or 13C nuclei.

This paper introduces a non-invasive NMR method to study multiple components in biomolecular condensates without tags, demonstrating its effectiveness with FUS and hnRNPC1 samples.

This study uses in-cell NMR at 310K to analyze α-synuclein and tau conformations and interactions in live human cells at micromolar concentrations.

The paper identifies SU(1,1) pair dynamics in brain proton spin ensembles using a symmetry-based framework and reanalysis of magnetic-resonance data.

This paper improves NMR spectroscopy for studying PET-degrading enzymes by recording spectra at higher temperatures, enhancing signal-to-noise ratios and enabling faster backbone assignment.

This paper uses proton-detected solid-state NMR to determine the high-resolution structure of MscL in native E. coli membranes, achieving better sensitivity than 13C-detected methods.

This study investigates oral administration of deuterated choline for metabolic imaging of brain tumors, showing comparable tumor contrast to intravenous administration using a clinically relevant dose.

This paper uses solid-state NMR to selectively detect rigid regions in chemotaxis signaling protein complexes, revealing conformational changes between signaling states.

The paper introduces a spectral shearing method to suppress diagonal peaks in NMR spectra while preserving cross peak sensitivity.

This paper introduces a 1H NMR method to enhance 13C signal detection for studying cellular metabolism in low-volume samples, demonstrating its application in tracking metabolic reactions in Clostridioides difficile.

NMRFx is a new software tool that integrates NMR data processing, visualization, analysis, and structure calculation, with case studies demonstrating its application in various molecular systems.

The paper introduces a 19F-based DNP MAS NMR method for atomic-level structural analysis of proteins in mammalian cells, demonstrating high sensitivity and specificity for detecting target proteins.

The paper reports high-resolution 13C and 15N backbone chemical shift assignments for Toho-1 β-lactamase using advanced solid-state NMR techniques, confirming conserved secondary structure.

The paper demonstrates a method to quantify small molecule partitioning between biomolecular condensate phases using 2D NMR spectroscopy without isolating components.

This study improves solid-state NMR resolution for high molecular weight proteins using GHz-class spectrometers and novel techniques, achieving better than 0.2 ppm resolution for proteins up to 144 kDa.

This study introduces a non-invasive NMR method to measure sodium ion transport in living cells, showing its ability to distinguish physiological states and correlate with metabolic activity.

This study uses solid-state NMR to analyze site-specific RNA dynamics through 15N T1 relaxation times, revealing correlations with structural elements.

The paper reports NMR resonance assignments for EmrE, a multidrug transporter, using solution and solid-state NMR techniques at specific pH conditions.

This paper introduces a microfluidic chip integrated with an NMR probe for real-time metabolic flux analysis of adherent cells, demonstrating its use with HeLa cells over 48 hours.

The paper develops an adaptive optimization system for NMR CEST experiments to improve estimation of minor protein conformational states using Bayesian methods and MCMC.

This paper designs 19F NMR labels to improve chemical shift dispersion and enable structural analysis of proteins and their interactions.

This study develops hyperpolarized silicon particles functionalized with MUC1 antibody for targeted molecular MRI of colorectal cancer, showing active tumor targeting in vivo.

This paper introduces a method to control pH in NMR samples using light-activated photoacids, enabling non-invasive and precise pH modulation for studying protonation states in proteins.

This study determines the structure of Mycobacterium tuberculosis CrgA, revealing its dimeric form and interactions with divisome proteins essential for cell division.

This study investigates E. coli metabolism on pyruvate as the sole carbon source for NMR studies, revealing metabolic inefficiencies that impact isotopic labeling.

The paper develops a cost-effective method for producing isotopically-labeled proteins in E. coli by optimizing nutrient use and reducing 13C-D-glucose consumption.

The paper provides a rigorous theoretical interpretation of the transverse spin relaxation rate {Gamma} 2, showing it is proportional to norm and offers an explicit formula for calculating norm without adjustable parameters.