Advanced NMR Techniques and Applications

The paper provides a rigorous theoretical interpretation of the transverse spin relaxation rate {Gamma} 2, showing it is proportional to norm and offers an explicit formula for calculating norm without adjustable parameters.

This paper introduces a Hadamard encoding method for multi-dimensional solid-state NMR spectral editing of protein sidechains, enabling unambiguous detection of signals from basic amino acids and their bound water molecules.

This study uses DNP-enhanced ssNMR to rapidly characterize native bacterial biofilms without labeling, identifying polysaccharides, proteins, and eDNA.

This paper develops a deep neural network, FID-Net-2, combined with custom NMR experiments to accurately reconstruct high-resolution aromatic 1H-13C correlation spectra from uniformly 13C-labeled protein samples.

This study evaluates AsymPolPOK's stability and performance as a polarization agent in mammalian cells, showing its effectiveness with electroporation but limitations due to rapid reduction in the cellular environment.

This study determines the structure of bacteriophage P22 scaffolding protein within its procapsid using magic-angle spinning NMR, revealing a trimer of dimers with both helical and disordered regions.

This study uses high-resolution 2D solid-state NMR to analyze native nontuberculous mycobacteria, identifying cell-wall chemical sites and structural differences between strains.

This paper develops an NMR method to measure dynamics in the fd bacteriophage coat protein, revealing helical motion and flexible regions.

This study uses solid-state NMR to investigate the conformational dynamics and interactions of high-molecular weight hyaluronic acid in a hydrated environment.

The paper identifies fluoride contamination from glass NMR tubes and proposes a simple solution to mitigate it in biomolecular applications.

This paper improves the accuracy of exchange parameters in CEST experiments by using K instead of kex to guide B1 field selection, demonstrating better results in two FF domain mutants.

This paper demonstrates a method to overcome the re-protonation bottleneck in NMR by using deuterated cell lysates, enabling high-resolution amide and Hα MAS NMR with improved sensitivity.

The paper demonstrates that deuteration via deuterated amino acids improves spectral resolution for large proteins in solid-state NMR by avoiding proton back-exchange issues.

This paper demonstrates long-lived coherences (LLCs) in proteins, enabling stereospecific detection of magnetic interactions through nuclear spin transitions in glycine residues.

The paper introduces SpecDB, a relational database for archiving NMR FID data and metadata, to support machine learning in biomolecular NMR analysis.

The paper presents a workflow combining CCPNMR2.4 and ARIA2.3 to handle N-terminal acetylation and C-terminal amidation in protein structure calculation, showing no significant difference from existing methods.

Interact automates analysis of 1D and 2D NMR titration experiments to quantify protein-ligand interactions through peak picking, annotation, and fitting procedures.

The paper describes the synthesis and purification of 5,5,5-trifluoroisoleucine and its perdeutero and 5-13C variants, along with 2-13C-trifluoroacetate, for use as precursors in introducing the 13CF3 group into proteins.