Barrier Structure and Function Studies

This study shows that astrocytes from 3xTG-AD mice impair BBB integrity in vitro through paracrine mechanisms, as evidenced by reduced tight junction protein expression and TEER.

This study shows that low-dose methamphetamine enhances drug transport across the blood-brain barrier via caveolar pathways, improving therapeutic efficacy in a glioblastoma model.

This study identifies a TRA2A/LINC00662/ELK4 axis that regulates BBB permeability in Alzheimer's disease by affecting ELK4 mRNA stability.

This study investigates how acute systemic inflammation affects neurovascular unit function and cerebrovascular dynamics in rats, showing altered haemodynamics and NVU pathology.

The paper identifies ECAL-1 as a regulator of cerebrovascular homeostasis and shows it controls vascular network formation through the miR-23a-Cldn5b axis in zebrafish.

This study shows that oral antibiotics alter gut microbiota in rhesus monkeys, increasing BBB permeability, particularly in the thalamus, potentially linking gut microbiota changes to neurological effects.

This study shows that reduced TNAP enzyme activity in cerebral microvessels during late sepsis is linked to neuroinflammation and behavioral changes like anti-nociception.

This study shows that apoM-bound S1P regulates BBB permeability and transcytosis, with S1PR1 agonists able to restore permeability in apoM-deficient mice.

The paper demonstrates that Plvap regulates the rate of blood-borne protein passage through fenestrated endothelia in the hypophysis using zebrafish models.

The paper shows the hippocampus has lower oxygenation and weaker blood flow control than cortex due to vascular differences, linking this to its vulnerability in neurological diseases.

This study shows liposomes can selectively enter the brain after stroke through two distinct BBB disruption phases, offering new therapeutic opportunities for targeted drug delivery.

This study identifies transcriptomic changes in mouse ischemic cerebral microvessels and finds shared alterations with human stroke data, highlighting pathways relevant for preclinical research.

The paper investigates how pericyte and endothelial cell connectivity mediates neurovascular coupling, finding directional vasomotor responses and disrupted connectivity in diabetes.

This study shows that insulin receptors in astrocytes are necessary for brain uptake of circulating insulin and glucose.

This study shows glucose exposure harms the neurovascular unit and behavior in zebrafish, but sodium nitroprusside reverses these effects.

The paper studies zebrafish blood-brain barrier development, finding that suppression of transcytosis is critical for BBB formation and that Mfsd2a plays a conserved role in this process.

The paper investigates the development of vascular myogenic responses in zebrafish, showing that pericytes and vascular smooth muscle cells regulate vascular tone at different developmental stages.

This study shows that amyloid β oligomers constrict human capillaries via pericyte signaling, reducing blood flow and contributing to Alzheimer’s disease pathology.

The paper describes the discovery of transient NO-containing spherical structures in zebrafish cerebral endothelial cells during development, showing their dependence on Notch signaling.

This study identifies precapillary sphincters as regulators of cerebral blood flow, showing their dilation and constriction in response to neural activity and ischemia.

The paper generates human pluripotent stem cell-derived brain pericyte-like cells that induce blood-brain barrier properties in endothelial cells and form an isogenic NVU model.

This study shows BBB dysfunction in aging leads to TGF-beta hyperactivation in astrocytes, causing neural dysfunction, which can be reversed by inhibiting TGF-beta signaling.

This study identifies macrophages as key components of the blood-nerve barrier (BNB) that reinforce the barrier by engulfing leaked material, contrasting with the blood-brain barrier (BBB) which relies more on endothelial cells.

This study uses single-cell RNA sequencing to show that brain endothelial cells respond to circulatory cues of aging, with aged plasma inducing aging transcriptomes and young plasma reversing them.

The paper demonstrates that loud music and sound can open the blood-brain barrier, offering new insights into BBB mechanisms and non-invasive drug delivery.

The paper identifies perivascular interneurons and fibro-vesicular complexes in rodent and rabbit brains, suggesting they function as mechanoreceptors within the neurovascular unit.

The paper describes the role of endothelial cell filopodia in the anastomosis of CNS capillaries using electron microscopy and Golgi methods.

This study identifies NOX5 as a key mediator of calcium-dependent reactive oxygen species formation and blood-brain barrier breakdown after reperfusion in stroke, suggesting NOX inhibition as a potential therapeutic strategy.

This paper presents an optimized method to isolate cerebral microvessels from mouse brains without enzymatic digestion, enabling consistent molecular characterization and analysis of gene expression, DNA methylation, and chromatin structure.