Brucella: diagnosis, epidemiology, treatment

The paper reanalyzes public transcriptomic and metabolomic data to prioritize antigens for improved Brucella vaccines by integrating single-cell and metabolomic atlases.

This study uses a CRISPR screen to identify host genes critical for early Brucella infection in human macrophage-like cells, revealing shared and pathogen-specific dependencies.

This study models the T4SS structure of Brucella melitensis and identifies three FDA-approved drugs that could target the system for anti-virulence therapy.

This study identifies Brucella PrpA as a protein that activates the Tpl2-ERK pathway to induce IL-10 secretion in macrophages, promoting chronic infection and suggesting the prpA mutant as a potential vaccine.

The paper develops a multi-host model for brucellosis in the French Alps, identifying Alpine ibex and their environment as essential for pathogen maintenance and transmission to livestock.

This study evaluates inactivation methods for Brucella melitensis using Astral-DIA proteomics, finding that heat and formaldehyde affect antigenicity and protein expression differently across strains.

This study evaluates a DNA vaccine against Brucella abortus using chitosan-modified PLGA nanoparticles in mice, showing enhanced immunogenicity and protective efficacy compared to naked DNA.

This study identifies an actin-dependent mechanism by which Brucella abortus exits host cells in infective clumps, forming extracellular vesicles that can reinfect neighboring cells.

This study identifies three modes of Brucella abortus exit from host cells, including cell-to-cell transmission, which may enhance immune evasion and persistence.

This study shows that Brucella-induced spondyloarthritis in SKG mice results from both persistent bacterial infection and host immune reactivity, with species-specific differences in disease progression.

This study analyzes metabolomic changes in murine tissues infected with Brucella melitensis, finding significant metabolic alterations in reproductive tracts, spleens, and livers at two weeks post-infection.

This study quantifies growth rates of Brucella species in vitro and intracellularly, revealing species-specific differences despite high genomic similarity.

This study identifies BspD as critical for maintaining Brucella envelope integrity under stress, showing its role in bacterial survival within host cells.