bioluminescence and chemiluminescence research

This study shows that firefly luciferase phase separates into crystal-like inclusions at lower temperatures, which is suppressed by D-luciferin and stabilizing inhibitors.

This paper designs a smaller fusion protein for H. pylori detection using epitopes, improving recombinant production and detection capabilities.

The paper develops a whole-cell biosensor for ethylene oxide and ethylene using genetic parts from Mycobacterium, achieving detection limits of 0.2 μM for ethylene oxide and detecting ethylene from banana fruit.

This paper develops a bioluminescent indicator for potassium ions using a modified luciferin and engineered luciferase, validated in cell lines, neurons, and live mice.

The paper develops new luciferin substrates that enhance bioluminescence brightness and stability, enabling high-speed video-rate imaging of mice with improved sensitivity and non-invasive monitoring of calcium activity.

This paper introduces Finding Fluorescence, a community science platform that documents novel biofluorescence occurrences and highlights the ecological roles of fluorescence across species.

The paper develops an in vitro method to optimize imaging parameters for bioluminescence-based neuronal activity recording without animal use.

The paper identifies 16 candidate genes and 12 enriched GO terms potentially involved in bioluminescence in scale worms using RNA-seq and histological analyses.

The paper engineers a ratiometric bioreporter in Pseudomonas synxantha 2-79 to detect phosphorus limitation in soil, showing improved performance over native promoters and robustness across pH ranges.

This study uses generative modeling and biochemistry to improve luciferase activity and stability by engineering its active center and scaffold, achieving a 50% success rate for single mutants.

The paper develops a high-throughput, quantitative bioluminescence reporter system for plant synthetic biology with improved dynamic range and application in synthetic gene circuit optimization.

LucFlow measures Luciferase reporter expression in single cells with higher accuracy than bulk methods by excluding dead cells.

The paper develops a bioluminescent genetically encoded glutamate indicator for in vivo neuronal activity monitoring without requiring external light sources.

This study models and analyzes a luciferase-like monooxygenase from Pseudomonas meliae, identifying mutations that prevent bioluminescence and suggesting ways to restore its function.

This paper expands luciferase reporter systems for cell-free protein expression by introducing two orthogonal luciferases and a substrate regeneration pathway.

This paper introduces a bioluminescent platform for tracking RNA in vivo, demonstrating single-copy detection and robust imaging in cells and live animals.

This paper develops a semi-automated pipeline using machine learning to improve ATP measurement accuracy in nerve terminals from bioluminescence images.

This study demonstrates that gas-reporting biosensors can detect gene expression in environmental soil matrices with minimal perturbation by optimizing hydration and biosensor titer.

This paper introduces a method using real-time luminescence to estimate drug response in cell lines, avoiding the need for cell counting and fluorescent labeling.

LuminoCell is an affordable, open-source luminometer platform for real-time monitoring of in-cell luciferase activity in various biological assays.

The paper develops a whole-cell eukaryotic copper biosensor in yeast with improved sensitivity and specificity for detecting bioavailable copper at low concentrations.

This paper introduces LuCID, a tool that detects calcium in real-time and converts it into stable gene expression, with applications beyond calcium signaling.

This paper develops a bioluminescent calcium indicator and prosubstrate for non-invasive imaging of neuronal activity in awake mice.

The paper presents the first draft genome assembly of Phrixothrix hirtus, revealing gene family expansions and bioluminescence-related genes shared with Elateroidea.

The paper introduces AMBER, a genetically encoded bioluminescent voltage indicator that does not require exogenous substrate, enabling voltage imaging in freely moving animals.

PlotXpress is a webtool that normalizes and visualizes reporter expression data using internal controls and provides standardized, publication-grade plots.

The paper develops a Golden Gate assembly system for constructing Escherichia coli bioreporters that detect mercury and arsenic under aerobic and anaerobic conditions.

The paper develops a dual-color luciferase reporter system for faster and more consistent characterization of transcriptional regulatory elements in plants.

The paper investigates the 'flash' kinetics of Gaussia Luciferase, showing that the decay in light signal is due to irreversible enzyme inactivation caused by covalent substrate binding.

The paper sequenced genomes of five bioluminescent Mycena fungi to study the evolution of luciferase clusters and bioluminescence, finding that the trait originated in a common ancestor and was lost in some lineages.