bioluminescence and chemiluminescence research

The paper investigates the 'flash' kinetics of Gaussia Luciferase, showing that the decay in light signal is due to irreversible enzyme inactivation caused by covalent substrate binding.

The paper sequenced genomes of five bioluminescent Mycena fungi to study the evolution of luciferase clusters and bioluminescence, finding that the trait originated in a common ancestor and was lost in some lineages.

This paper reports an in silico analysis of luciferase in Mycobacterium sp. EPa45, comparing its physiochemical, structural, and phylogenetic characteristics to other mycobacterial and bacterial strains.

The paper designs modular biosensors using de novo protein switches that detect various analytes with high sensitivity and modularity.

This paper reports the first complete life cycle and mitochondrial genome of the luminous ostracod Vargula tsujii, enabling future studies on bioluminescence biosynthesis and genomic manipulation.

This paper develops a ratiometric bioluminescent biosensor to image cyclic di-GMP levels in bacteria during host infection.

This paper designs a cell-free biosensor for atrazine detection using a metabolic pathway and a cyanuric acid biosensor, achieving faster and tunable detection compared to whole-cell sensors.

This paper develops a multiplex hextuple luciferase assay to simultaneously assess multiple cellular pathways and demonstrates its application in monitoring pathway responses to various treatments.

The paper develops a bacterial biosensor system in C. elegans to detect environmental changes in the intestinal tract using an inducer.

This paper introduces a NanoLuc-based method for highly sensitive gene expression detection in C. elegans, showing it can detect signals at 400,000-fold over background in populations and from single worms.

The paper introduces a regression-based normalization method for Luciferase reporter data, showing it outperforms ratiometric normalization, especially at low transfection efficiency.

The paper generates genomes for three bioluminescent beetles to study the genetic basis of bioluminescence and finds at least two independent evolutionary origins of the trait.

This paper develops two bacterial biosensors that detect and quantify silver and copper ions using GFP fluorescence, showing correlation between ion concentration and signal intensity.

This paper develops a modeling approach to infer gene transcription levels from Lux bioluminescent data, addressing nonlinear dynamics that affect light output.