Animal Genetics and Reproduction

The paper uses PacBio HiFi sequencing to determine the genomic integration site and structure of the Nestin-CreERT2KEisc/J transgene, revealing deletions and enabling zygosity testing.

The paper introduces the Cell Viability and Utility Index (CVUI) to assess cell culture quality in wildlife biobanking, validated across 154 culture rounds from 46 species.

This paper demonstrates a method to induce male-to-female sex reversal in mice using Y-chromosome elimination and somatic cell nuclear transfer, enabling reproduction from a single male genome.

This paper uses CRISPR/Cas9 to edit bovine satellite cells to remove the alpha-gal epitope, creating AGS-compatible cultivated meat with reduced allergenic potential.

This paper reports the first successful cloning of a golden wild yak using interspecific somatic cell nuclear transfer under extreme high-altitude conditions.

This study develops a method using bovine epiblast stem cells for efficient gene editing and cloning of cattle, resulting in higher birth and survival rates compared to fibroblast-based cloning.

The paper shows that inhibiting ATM with AZ32 improves AAV-mediated long-fragment knock-in efficiency in pig zygotes, enabling double knock-in at two loci.

This study improves SCNT efficiency in cattle by delaying oocyte maturation and using biPSCs as donor nuclei, resulting in higher fusion and blastocyst rates.

This study constructs and evaluates a GnRH vaccine using MT3, showing it induces antibodies and reduces hormone levels in mice, leading to testicular changes associated with immune castration.

The paper reports the successful cloning of a hybrid yak-cattle (Pien-niu) via interspecies somatic cell nuclear transfer at an ultra-high-altitude region, demonstrating its viability for in situ conservation.

The paper describes a method to achieve targeted sex ratio distortion in mice using a selfish supergene component, resulting in a high prevalence of non-transgenic offspring of the desired sex.

This paper establishes a method to generate humanized mice with targeted gene knockouts in the human immune system using Cas9-edited CD34+ cells.

This study develops a method to produce pseudopregnant female mice using non-estrous individuals and the Whitten effect, achieving high copulation and embryo transfer success rates.

The paper compares trans-chromosomic rodent models and finds rats show improved somatic hypermutation and class-switch recombination compared to mice.

Researchers cloned an endangered black-footed ferret using cross-species somatic cell nuclear transfer, producing three healthy kits to aid genetic rescue.

This study improves transgenic mouse production efficiency using piggyBac, enabling phenotyping in the founder generation and demonstrating application with bacterial artificial chromosome transgenes.

The paper analyzes the Ucp1-CreEvdr transgenic mouse line and finds it causes complex developmental and metabolic issues independent of its intended genetic manipulation.

The paper investigates chromatin reprogramming during embryonic genome activation in bovine embryos, finding that SCNT embryos fail to reprogram certain EGA gene targets and that DUXC over-expression can rescue this defect.

This study measures hematology and serum parameters in Venus transgenic rabbits to assess the impact of the fluorescent protein on hematopoiesis and general health.

The paper develops a method to identify transgenic marmoset embryos using a fluorescent protein indicator system based on the piggyBac transposon.

This study demonstrates that a circular human artificial chromosome can be transmitted through three generations of mice without health or reproductive issues.

This study identifies Alyref and Gabpb1 as developmentally required genes whose incomplete activation causes preimplantation arrest in cloned mouse embryos.

The paper presents a MapCap sequencing workflow to locate and count transgenic sequences in mice, identifying insertion sites and characterizing genetic variations in a diabetes model.

The paper develops an R package and website to optimize breeding design for gene-modified animals, minimizing animal use while ensuring successful outcomes.

This study successfully transplants spermatogonia between marine fish species in the Pleuronectiformes order, achieving high efficiency and producing donor-derived offspring.

The paper derives and characterizes XEN cells from pig embryos, showing their ability to contribute to chimerism and serve as nuclear donors for cloning.

This paper generates transgenic quails using plasmid-based in vivo transfection of primordial germ cells, overcoming limitations of previous viral methods.

The paper introduces a transposon-based system for genetic manipulation of the vertebrate CNS, enabling long-term conditional experiments across cell types and species.

This study quantifies CMV promoter activity in various porcine tissues using a transgenic pig model expressing ZsGreen1, revealing tissue-specific expression patterns.

The paper describes an immunosurgery method to effectively ablate endogenous primordial germ cells in chick embryos, enhancing chimerism rates when grafting foreign PGCs.