Seiya Mizuno

This study uses a Col22a1-EGFP reporter to visualize and analyze the dynamic three-dimensional structure of the myotendinous junction across development and in response to mechanical stress.

This paper creates a mouse model with a patient-derived LMX1B variant, showing tissue-specific effects of nail-patella syndrome.

This study shows that continuous nuclear export of p62 is essential for kidney homeostasis, as its disruption leads to renal failure due to excessive nuclear accumulation and aggregate formation.

The paper introduces GT-SPARCL, a genetic method for low-density neuronal labeling in mice, and applies it to reveal morphological subtypes of AVP neurons in the SCN.

This study identifies Tff2+ progenitors in the gastric corpus as the primary source of SPEM and gastric cancer following injury, showing they can bypass metaplasia and progress directly to dysplasia.

TSUMUGI identifies gene networks driven by phenotypes using IMPC knockout mouse data to facilitate hypothesis generation for complex phenotypes.

The paper traces individual PGC clones in mice to show early stochastic bottlenecks shape clonal contributions and reproductive success.

This study examines the spatiotemporal expression of CD73 in the mouse retina across development and adulthood, revealing its role in retinal function and adenosine signaling.

This paper introduces a novel genetic mouse model of aortic dissection showing immune cell infiltration and altered ECM dynamics.

This study identifies Muc6-expressing isthmus progenitors as key contributors to gastric regeneration and metaplasia through myeloid-mesenchymal interactions.

KOnezumi-AID automates gRNA design for Target-AID-mediated gene knockouts, enabling efficient and safe multiplex knockout strategies in mouse and human genomes.

The paper identifies Dusp13 and Dusp27 as MYOD target genes that regulate muscle stem cell transition from proliferation to differentiation during myogenesis.

The paper introduces a novel method for functional analysis of non-coding regions by inducing random mutations in target genomic regions using CRISPR and transposase in mouse embryonic stem cells.

This study improves transgenic mouse production efficiency using piggyBac, enabling phenotyping in the founder generation and demonstrating application with bacterial artificial chromosome transgenes.

This study generates functional adult hearts in Mesp1/2-deficient mice using blastocyst complementation with pluripotent stem cells, showing successful derivation of all cardiovascular lineages from exogenous cells.

This paper introduces a universal method for generating knockout mice in multiple inbred strains using zygote electroporation, enabling efficient phenotyping for gene function and disease research.

This study shows that Max gene disruption in germ cells leads to meiotic onset but results in apoptosis due to stalled meiotic progression.

This study identifies that MeApd-ERβ+ neurons regulate receptivity-based preference in male mice but not sex-based preference.

The paper uses engineered human myoblasts to show that D4Z4 heterochromatin disruption and DUX4 network activation are key in FSHD.

This paper develops a chemogenetic method to selectively activate mGlu1 receptors in brain tissue using a palladium complex and a mutant receptor, demonstrating its role in synaptic plasticity.

This study shows that reducing ooplasmic volume in oocytes improves meiotic accuracy and embryo development, enabling offspring production from azoospermic mice with spermatocyte arrest.

This paper introduces DAJIN, a machine learning-assisted long-read sequencing method for comprehensive multiplex genotyping of genome editing outcomes, including intended and unintended mutations.

This study identifies MAST4's role in regulating DLX3 and stem cell maintenance in mouse incisor development, leading to Amelogenesis Imperfecta.

The paper identifies cell-type specific genes in olfactory bulb projection neurons using single-cell RNA-seq and validates them as Cre-driver markers for genetic labeling.

This study identifies EXOC1's role in regulating pseudopod formation in spermatogonia and syncytium formation in spermatocytes in mice, highlighting its interaction with Rac1 and SNAP23/STX2.