Discover, rate, and discuss biology preprints.

This paper evaluates multi-eGO's ability to predict PDZ2-peptide binding changes across mutations, showing good agreement with experimental data for dissociation constants but limited success in capturing kinetic effects.

This paper introduces multistate enzyme design to optimize active sites across reaction coordinates, achieving higher catalytic efficiency and enantioselectivity than single-state designs.

This study presents cryo-EM structures of SV2C and its ligands, revealing structural differences that explain ligand selectivity and impact dopamine release.

This study shows that substrate binding, not tRNA or ribosome interactions, activates GCN2 kinase by relieving autoinhibition and promoting autophosphorylation.

This study develops ADAMTS13 mimetics that restore VWF cleavage and prevent thrombocytopenia in iTTP mice by using antibody-mediated substrate targeting.

PANDA generates high-quality all-atom protein structures by denoising atomic occupancy patterns, achieving high self-consistency and scaffolding success on functional design tasks.

This study quantifies the interaction between full-length human calcineurin and NHE1-CnBD under different activation states and shows NHE1 inhibits substrate dephosphorylation.

This paper identifies a new class of inherently efficient SUMOylation substrates by showing that ZBTB38BTB and related BTB domains can be SUMOylated efficiently without E3 ligase involvement.

This paper identifies a novel non-canonical RT-loop degron in VAV1 and demonstrates how conformational restriction and computational modeling enhance molecular glue potency for proteasomal degradation.

This study determines cryo-EM structures of MeCP2 bound to nucleosomes and identifies how RTT mutations affect nucleosome interactions and chromatin targeting.

Fluxion generates enzyme function predictions by modeling electron flow trajectories, enabling function generation beyond static classification.

This study shows myosin-X can bundle actin filaments and regulate barbed-end dynamics, suggesting it can initiate filopodia without passive crosslinkers.

This study shows that a single RNA evolution experiment generated multiple distinct ligase ribozymes with diverse reactivities, highlighting RNA's catalytic flexibility and evolutionary potential.

This study compares protein composition between gluten-free and barley-malt beers, finding lower protein levels and foam-active proteins in gluten-free beers.

This paper evaluates three eukaryotic cell-free translation systems to determine their suitability for assessing mRNA translatability in vaccine development.

The paper identifies covalent molecular glue inhibitors that stabilize a non-productive Kalirin-Rac1 complex, inhibiting nucleotide exchange and reducing GEF turnover.

The paper introduces an anesthesia-free infusion technique for in vivo metabolic tracing in mice, achieving steady-state labeling comparable to jugular infusion without excessive cystine accumulation.

This study uses deep mutational scanning to analyze a phage tailspike protein, revealing structural constraints and adaptive flexibility that influence its function and host range.

This study shows directed evolution improves a designed enzyme by enhancing its transition-state-like conformation, reducing reaction barriers through desolvation and electric field organization.

The paper identifies a hydrogenase-like activity in mitochondrial Complex I under hypoxia in vascular plants, linking it to NADH cycling and potential ROS reduction.