Roberto A. Chica

This paper introduces multistate enzyme design to optimize active sites across reaction coordinates, achieving higher catalytic efficiency and enantioselectivity than single-state designs.

The paper designs de novo TIM barrels with structural lids to create active sites for enzymatic function, achieving high catalytic efficiency.

This study shows how endolysins adapt predictably through mutations that enhance substrate recognition, despite functional trade-offs and some variability in evolutionary paths.

This study uses NMR peak intensity analysis to identify functional dynamics hotspots in proteins, showing that specific structural flexibility correlates with function in red fluorescent proteins.

This study shows that distal mutations improve enzyme catalysis by enhancing substrate binding and product release, working with active-site mutations to increase overall activity.

The paper investigates how distal mutations in a retro-aldolase affect loop dynamics and catalytic efficiency, showing an epistatic effect when combined with active-site mutations.

This paper designs artificial enzymes with high catalytic efficiency using crystallography-enhanced computational methods, achieving improvements comparable to directed evolution.

This paper introduces a genetically-encoded fluorescent biosensor that detects protein expression in live cells within seconds, significantly faster than standard fluorescent protein fusions.

ProtaBank is a database for storing and sharing protein design and engineering data, offering tools for analysis and benchmarking predictive models.