Discover, rate, and discuss biology preprints.

The paper develops a multilocus sequence analysis scheme to resolve diversity within the X-disease phytoplasma group using genome data, identifying four lineages and demonstrating limitations of 16S rRNA RFLP classification.

This study identifies stage-specific and biphasic miRNA-mRNA network reorganization in preeclampsia using longitudinal RNA sequencing and experimental validation.

The paper uses ChromBPNet to predict chromatin accessibility effects of noncoding variants across hematopoietic cell types, finding lineage-structured patterns and complementary insights with GWAS fine-mapping.

This paper optimizes dCas12a-based CRISPRi/a for pooled screening in Yarrowia lipolytica, identifying effective crRNA features and a high-performing effector variant.

DeepRNAi predicts siRNA on- and off-target activity using deep learning, showing high accuracy across species and genomes, and highlights off-target concerns in potent siRNAs.

The paper identifies an oxidative mutational signature in normal tissues of individuals carrying a single pathogenic MUTYH allele, suggesting a measurable mutational risk in healthy tissues.

This study develops sctRNA-seq to profile mRNA and tRNA in single cells, revealing tRNA regulation dynamics and their role in cellular identity and stress responses.

This study presents a multi-omic analysis of the bent-wing bat genome to explore immune-related adaptations and viral interactions.

MicroDUET is a genomic foundation model that provides fast, accurate representations for pathogen surveillance by distinguishing phylogeny and function.

The paper presents a chromosome-level genome assembly for the northern dragonhead plant, providing a high-quality resource for future research and conservation.

This study benchmarks DNA polymerases for long-read sequencing and genome assembly, finding that combining polymerases improves assembly quality and variant calling accuracy.

This study uses sedimentary ancient DNA to expand bioarchaeological insights at Roman Harelbeke, revealing broader biological signals and environmental taxa not captured by traditional methods.

The paper uses long-read sequencing to analyze human 5S rDNA arrays, revealing zoned methylation patterns linked to chromatin structure.

This paper uses oyster genomics to identify genomic regions associated with size and immune response, highlighting genome structure's role in environmental adaptation and health.

This study uses saturation barcoding and ultra-long-read sequencing to resolve the structure and variation of human nucleolar organizer regions (NORs) in the K562 cell line.

The paper presents a chromosome-level genome assembly for the endangered northern dragonhead sap beetle, providing a genomic resource for conservation.

This study uses whole-genome sequencing data from 26 Finnish golden eagles over 136 years to show drastic genetic erosion post-bottleneck despite population recovery.

This study uses single-nucleus long-read RNA sequencing to identify isoform usage shifts in mouse kidneys during acute injury, revealing changes in gene expression and isoform composition.

The paper evaluates genomic methods to detect outbreaks of a vancomycin-resistant Enterococcus faecium lineage, finding that lower allelic thresholds improve outbreak detection.

This paper presents a chromosome-scale genome of Spondias tuberosa, revealing conserved genome architecture and gene family expansions in Anacardiaceae.