A. Asokan

Repurposing CRISPR-Cas13 systems for robust mRNA trans-splicing

This study repurposes CRISPR-Cas13 systems to enable trans-splicing of exogenous RNA fragments into endogenous pre-mRNA, improving editing of large mRNA segments in mammalian cells.

Rescuing AAV gene transfer from antibody neutralization with an IgG-degrading enzyme

This study shows that administering an IgG-degrading enzyme (IdeZ) can overcome pre-existing antibodies against AAV vectors, improving gene therapy efficacy in animal models.