A. Pavao

HRMAS 13C NMR and genome-scale metabolic modeling identify threonine as a preferred dual redox substrate for Clostridioides difficile

Central in vivo mechanisms by which C. difficile's proline reductase drives efficient metabolism, growth, and toxin production

This study shows that proline reductase in C. difficile is essential for metabolism, growth, and toxin production in vivo, as its absence delays colonization and disease progression in mice.

Elucidating dynamic anaerobe metabolism with HRMAS 13C NMR and genome-scale metabolic modeling.

This study uses HRMAS 13C NMR and genome-scale metabolic modeling to analyze dynamic anaerobic metabolism in Clostridioides difficile, revealing metabolic integration of glycolytic and amino acid fermentation pathways.

Clostridium scindens colonization of gnotobiotic mice promotes a chronic unresolving infection with Clostridioides difficile

This study shows that C. scindens colonization in gnotobiotic mice reduces acute C. difficile infection severity but does not prevent chronic infection development.

Real-time HRMAS 13C NMR of obligately anaerobic cells identifies new metabolic targets in the pathogen Clostridioides difficile

This study uses real-time HRMAS 13C NMR to analyze metabolism in C. difficile, identifying new metabolic integration points and informing a genome-scale model.