J. Peltier

HRMAS 13C NMR and genome-scale metabolic modeling identify threonine as a preferred dual redox substrate for Clostridioides difficile

The TcdE holin drives toxin secretion and virulence in Clostridioides difficile.

This study shows that TcdE holin is essential for toxin secretion and virulence in C. difficile, as tcdE mutants exhibit reduced toxin release and less virulence in mice.

Central in vivo mechanisms by which C. difficile's proline reductase drives efficient metabolism, growth, and toxin production

This study shows that proline reductase in C. difficile is essential for metabolism, growth, and toxin production in vivo, as its absence delays colonization and disease progression in mice.

Elucidating dynamic anaerobe metabolism with HRMAS 13C NMR and genome-scale metabolic modeling.

This study uses HRMAS 13C NMR and genome-scale metabolic modeling to analyze dynamic anaerobic metabolism in Clostridioides difficile, revealing metabolic integration of glycolytic and amino acid fermentation pathways.

c-di-AMP signaling is required for bile salts resistance and long-term colonization by Clostridioides difficile

This study identifies c-di-AMP as a key signaling molecule in Clostridioides difficile that regulates osmolyte uptake and bile salt resistance, essential for gut colonization.

The E3 ligase RNF115 regulates phagosome maturation and host response to bacterial infection

This study identifies RNF115 as an E3 ligase that regulates phagosomal maturation and host response to bacterial infection through ubiquitylation.

Real-time HRMAS 13C NMR of obligately anaerobic cells identifies new metabolic targets in the pathogen Clostridioides difficile

This study uses real-time HRMAS 13C NMR to analyze metabolism in C. difficile, identifying new metabolic integration points and informing a genome-scale model.

Identification of RNAs bound by Hfq reveals widespread RNA partners and a sporulation regulator in the human pathogen Clostridioides difficile

The paper identifies RNAs bound by Hfq in C. difficile, revealing new RNA partners and a role for one ncRNA in sporulation regulation.

Ser/Thr kinase-dependent phosphorylation of the peptidoglycan hydrolase CwlA controls its export and modulates cell division in Clostridioides difficile

This study identifies CwlA as a substrate of the STK PrkC in C. difficile, showing that phosphorylation regulates its export and modulates cell division by controlling peptidoglycan hydrolysis.

Proteomics characterisation of the L929 cell supernatant and its role in BMDM differentiation

The paper characterizes L929 cell supernatant proteome and shows it induces a stronger anti-inflammatory M1 phenotype in BMDMs compared to M-CSF alone.

Type I toxin-antitoxin systems contribute to mobile genetic elements maintenance in Clostridioides difficile and can be used as a counter-selectable marker for chromosomal manipulation

The paper characterizes type I toxin-antitoxin systems in C. difficile prophages, showing their role in prophage stability and introducing them as counter-selectable markers for chromosomal manipulation.

Macrophage Scavenger Receptor 1 mediates lipid-induced inflammation in non-alcoholic fatty liver disease

This study identifies MSR1 as a key mediator of lipid-induced inflammation in NAFLD, showing its role in macrophage activation and potential as a therapeutic target.

The mechanisms of in vivo commensal control of Clostridioides difficile virulence

This study identifies how commensal bacteria influence Clostridioides difficile virulence through metabolic and regulatory mechanisms in a mouse model.

Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44

This study identifies novel SGK3 substrates, including endosomal proteins, through phosphoproteomic screens and shows SGK3 specifically phosphorylates these proteins at sites distinct from Akt.

Thermal proteome profiling of breast cancer cells reveals proteasomal activation by CDK4/6 inhibitor palbociclib

This study uses thermal proteome profiling to show palbociclib induces proteasome stabilization and senescence in breast cancer cells via ECM29 loss, with ECM29 mRNA levels linked to relapse-free survival.