B. Dupuy

The paper develops a monoclonal antibody collection targeting C. difficile strains to improve research on clinically relevant ribotypes.

This study identifies monoclonal antibodies targeting C. difficile's S-layer protein, revealing both protective and detrimental effects on bacterial physiology.

This study shows that TcdE holin is essential for toxin secretion and virulence in C. difficile, as tcdE mutants exhibit reduced toxin release and less virulence in mice.

This study shows that proline reductase in C. difficile is essential for metabolism, growth, and toxin production in vivo, as its absence delays colonization and disease progression in mice.

This study shows that extracellular succinate induces biofilm formation in C. difficile, leading to thicker, more complex biofilms associated with infection relapse.

This study identifies CD1687 as a DNA-binding lipoprotein required for DCA-induced biofilm formation in C. difficile by promoting cell-matrix interactions through eDNA binding.

This study uses HRMAS 13C NMR and genome-scale metabolic modeling to analyze dynamic anaerobic metabolism in Clostridioides difficile, revealing metabolic integration of glycolytic and amino acid fermentation pathways.

This study identifies c-di-AMP as a key signaling molecule in Clostridioides difficile that regulates osmolyte uptake and bile salt resistance, essential for gut colonization.

This study identifies bile as a trigger for biofilm formation in Bacteroides thetaiotaomicron and shows that extracellular DNA degradation by DNase BT3563 is essential for this process.

This study uses real-time HRMAS 13C NMR to analyze metabolism in C. difficile, identifying new metabolic integration points and informing a genome-scale model.

This study identifies extracellular pyruvate as a trigger for biofilm formation in C. difficile in the presence of DOC, revealing a mechanism for pathogen persistence in the gut.

The paper identifies RNAs bound by Hfq in C. difficile, revealing new RNA partners and a role for one ncRNA in sporulation regulation.

This study identifies CwlA as a substrate of the STK PrkC in C. difficile, showing that phosphorylation regulates its export and modulates cell division by controlling peptidoglycan hydrolysis.

The paper characterizes type I toxin-antitoxin systems in C. difficile prophages, showing their role in prophage stability and introducing them as counter-selectable markers for chromosomal manipulation.

This study identifies how commensal bacteria influence Clostridioides difficile virulence through metabolic and regulatory mechanisms in a mouse model.

This study identifies Zea, an RNA-binding protein secreted by L. monocytogenes, which stabilizes bacterial RNAs and activates RIG-I signaling to enhance interferon β production and modulate virulence.