Quantitative Profiling of Lysosomal pH Heterogeneity using Fluorescence Lifetime Imaging Microscopy
D. Deng, Y. Guan, B. Wang, H. Zheng, A. S. Mutlu, M. C. Wang
This paper introduces a FLIM-based method to quantitatively analyze lysosomal pH heterogeneity in mammalian cells and C. elegans, revealing pH differences and dynamics without transgenic reporters.
bioRxiv · Sep 25, 2023 · PreprintCell Biology