M. C. Wang

CRISPR/Cas14a Combined with RPA for Visual Detection of Marek's Disease Virus

The paper develops a rapid, instrument-free, and specific nucleic acid detection method for Marek's Disease Virus (MDV-1) using CRISPR/Cas14a and RPA, achieving a detection limit of 24.6 copies/L and 100% accuracy in clinical samples.

Organelle proteomic profiling reveals lysosomal heterogeneity in association with longevity

This study uses proteomic profiling to reveal lysosomal heterogeneity and its role in longevity by identifying protein dynamics and interactions in lysosomes.

FITM2 deficiency results in ER lipid accumulation, ER stress, reduced apolipoprotein B lipidation, and VLDL triglyceride secretion in vitro and in mouse liver

This study shows FITM2 deficiency reduces VLDL triglyceride secretion and causes ER stress and lipid accumulation in liver cells and mice.

Quantitative Profiling of Lysosomal pH Heterogeneity using Fluorescence Lifetime Imaging Microscopy

This paper introduces a FLIM-based method to quantitatively analyze lysosomal pH heterogeneity in mammalian cells and C. elegans, revealing pH differences and dynamics without transgenic reporters.

Quantification of glutathione with high throughput live-cell imaging

This study quantifies glutathione dynamics in living cells using a reversible fluorescent probe, demonstrating its sensitivity and applicability in high-throughput imaging.

Mitochondrial GTP Metabolism Regulates Reproductive Aging

This study shows that mitochondrial GTP metabolism regulates reproductive aging in C. elegans by controlling mitochondrial dynamics and homeostasis.

Lipid chaperone LBP-8 coordinates with nuclear factors to promote longevity in Caenorhabditis elegans

The paper identifies nuclear factors that interact with the lipid chaperone LBP-8 in C. elegans, showing that nuclear import is required for LBP-8's longevity-promoting effects.

Lysosome Lipid Signaling from the Periphery to Neurons Regulates Longevity

This study identifies a lysosomal lipid signaling pathway from fat to neurons in C. elegans that promotes longevity by regulating neuropeptide signaling.

Lysosomal nucleotide metabolism regulates ER proteostasis through mTOR signaling

This study identifies RSH-1 as a lysosomal NADPH phosphatase that regulates ER proteostasis via mTOR signaling in C. elegans.

Neuronal lipolysis participates in PUFA-mediated neural function and neurodegeneration

This study shows that neuronal lipolysis prevents lipid droplet accumulation and alleviates neurodegeneration in C. elegans by regulating PUFA partitioning and phospholipid incorporation.

Enhancing Intracellular Accumulation and Target Engagement of PROTACs with Reversible Covalent Chemistry

This study discovers that reversible covalent chemistry enhances PROTAC intracellular accumulation and target engagement, leading to improved efficacy.

Quantitative Real-Time Imaging of Glutathione with Sub-Cellular Resolution

This study develops a fluorescent probe to measure glutathione concentrations in the nucleus and cytosol, finding no significant concentration gradient between them.