Phasor histone FLIM-FRET microscopy quantifies spatiotemporal rearrangement of chromatin architecture during the DNA damage response.
J. Lou, L. Scipioni, B. K. Wright, T. K. Bartolec, J. Zhang, V. P. Masamsetti, K. Gaus, E. Gratton, A. J. Cesare, E. Hinde
The paper develops a biophysical method to quantify chromatin compaction dynamics during DNA damage response using FLIM-FRET microscopy and identifies roles of ATM and RNF8 in chromatin reorganization.
bioRxiv · Sep 17, 2018Biophysics