E. Hinde

Actin dynamics sustains spatial gradients of membrane tension in adherent cells

The paper demonstrates that adherent cells maintain spatial gradients of membrane tension through actin dynamics and membrane-cortex attachments, with tension lowest at adhesion edges and highest at protrusions.

Cutting through host autofluorescence: fluorescence lifetime imaging microscopy for visualising intracellular bacteria in Symbiodiniaceae

The paper uses fluorescence lifetime imaging microscopy to study the spatial association between B. minutum and intracellular bacteria under light-dark cycles, finding changes in bacterial abundance linked to photoperiod.

In silico and in vitro characterization of the mycobacterial protein Ku to unravel its role in non-homologous end-joining DNA repair

The paper characterizes the DNA binding properties of mycobacterial Ku (mKu) and its role in NHEJ using in silico and in vitro methods, showing its ability to stabilize DNA and protect termini from degradation.

A General Fluorescence-Based Method for Quantifying and Mapping Biomolecular Polarity In Vitro and In Cells

This paper introduces a fluorescence-based method to quantify and map biomolecular polarity in vitro and in cells, showing that polarity varies with phase separation types and stress conditions.

Proximity labelling identifies pro-migratory endocytic recycling cargo and machinery of the Rab4 and Rab11 families

The paper uses proximity labeling to identify protein complexes associated with Rab4, Rab11a, and Rab25 in cancer cells, revealing their roles in endocytic recycling and cell migration.

Live cell biosensors based on the fluorescence lifetime of environment-sensing dyes

This study develops a FLIM biosensor using merocyanine dyes to detect conformational changes in Cdc42, showing improved sensitivity at low activation levels compared to FRET-based methods.

A dominant-negative SOX18 mutant disrupts multiple regulatory layers essential to transcription factor activity.

This study investigates how a dominant-negative SOX18 mutant disrupts transcription factor activity by interfering with chromatin-binding dynamics and recruiting the wild-type interactome.

Nascent mutant Huntingtin exon 1 chains do not stall on ribosomes during translation but aggregates do recruit machinery involved in ribosome quality control

This study investigates how mutant Huntingtin exon 1 chains form inclusions in cells, finding that nascent chains do not stall on ribosomes but that ribosome quality control proteins are recruited into the aggregates.

Phasor histone FLIM-FRET microscopy quantifies spatiotemporal rearrangement of chromatin architecture during the DNA damage response.

The paper develops a biophysical method to quantify chromatin compaction dynamics during DNA damage response using FLIM-FRET microscopy and identifies roles of ATM and RNF8 in chromatin reorganization.