E. Gratton

Building Fluorescence Lifetime Maps Photon-by-photon by Leveraging Spatial Correlations

This paper introduces a doubly nonparametric framework for FLIM that learns fluorescent species and absolute lifetime maps while addressing spatial correlations and low photon counts.

Fluorescence Lifetime: Beating the IRF and interpulse window

This paper introduces BNP-LA, a Bayesian nonparametric method for analyzing fluorescence lifetime data with arbitrary lifetimes, and demonstrates its effectiveness using synthetic and experimental data.

Live cell biosensors based on the fluorescence lifetime of environment-sensing dyes

This study develops a FLIM biosensor using merocyanine dyes to detect conformational changes in Cdc42, showing improved sensitivity at low activation levels compared to FRET-based methods.

Epigenetic dysregulation from chromosomal transit in micronuclei

This study shows that chromosomal transit through micronuclei causes epigenetic dysregulation in cancer cells, leading to long-term chromatin accessibility abnormalities.

Spatial transcriptomics using combinatorial fluorescence spectral and lifetime encoding, imaging and analysis

This paper introduces MOSAICA, a spatial transcriptomics technology that combines spectral and lifetime encoding with machine learning to enable highly multiplexed in situ biomarker profiling.

In vivo macromolecular crowding is differentially modulated by Aquaporin 0 in zebrafish lens: insights from a nano-environment sensor and spectral imaging.

This study uses ACDAN and spectral imaging to show that Aquaporin 0 variants modulate macromolecular crowding and water dynamics in zebrafish lens.

Ultrafast phasor-based hyperspectral snapshot microscopy for biomedical imaging

This paper introduces ultrafast phasor-based hyperspectral snapshot microscopy for biomedical imaging, achieving 10-100x faster imaging speeds and enabling metabolic imaging of live 3D mouse tissues.

Biophysical properties of AKAP95 protein condensates regulate splicing and tumorigenesis

This study shows that AKAP95 protein condensates regulate splicing and tumorigenesis through their biophysical properties.

A Modular Microarray Imaging System for Highly Specific COVID-19 Antibody Testing

The paper introduces a low-cost, portable imaging system for specific COVID-19 antibody testing using microarrays, achieving performance comparable to expensive commercial readers.

Nanoscale distribution of nuclear sites analyzed by superresolution STED-ICCS

This paper introduces STED-ICCS, a method combining superresolution microscopy with image cross-correlation spectroscopy to quantify nanoscale spatial distribution of functional nuclear sites in MCF10A cells.

Phasor histone FLIM-FRET microscopy quantifies spatiotemporal rearrangement of chromatin architecture during the DNA damage response.

The paper develops a biophysical method to quantify chromatin compaction dynamics during DNA damage response using FLIM-FRET microscopy and identifies roles of ATM and RNF8 in chromatin reorganization.