B. Roberts

YAP dysregulation triggers hypertrophy by CCN2 secretion and TGFβ uptake in human pluripotent stem cell-derived cardiomyocytes

This study shows that YAP overactivation in HCM mutant cardiomyocytes drives hypertrophy via CCN2 secretion and TGFβ uptake.

Enhanced endogenous gene tagging in human induced pluripotent stem cells via AAV6-mediated donor delivery

This paper develops a method to efficiently insert fluorescent tags into genes not expressed in hiPSCs using AAV6 delivery, optimized CRISPR conditions, and ddPCR/ICE to minimize INDELs and isolate tagged clones.

Previous oil exposure alters oil avoidance behavior in a common marsh fish, the Gulf Killifish Fundulus grandis

This study shows that prior oil exposure in Gulf killifish reduces their ability to avoid oiled sediments, indicating sublethal sensory impairment despite survival.

Scarless gene tagging of transcriptionally silent genes in hiPSCs to visualize cardiomyocyte sarcomeres in live cells

The paper describes a CRISPR/Cas9 method to tag transcriptionally silent genes in hiPSCs with mEGFP, enabling visualization of sarcomeric proteins in live cardiomyocytes.

Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization

The paper describes a CRISPR/Cas9 method to tag endogenous proteins in human iPSCs, generating 10 tagged lines with quality-controlled clones for cell biology research.