D. Yi

Enhanced endogenous gene tagging in human induced pluripotent stem cells via AAV6-mediated donor delivery

This paper develops a method to efficiently insert fluorescent tags into genes not expressed in hiPSCs using AAV6 delivery, optimized CRISPR conditions, and ddPCR/ICE to minimize INDELs and isolate tagged clones.

Scalable primordial germ cell-like-cell platform for functional genomics identifies epigenetic fertility modifiers

This paper develops a scalable method to generate PGC-like cells and uses it to identify epigenetic factors affecting germline development.

General Capillary Endothelial Cells Undergo Reprogramming into Arterial Endothelial Cells in Pulmonary Hypertension through HIF-2α/Notch4 Pathway

E2F1 Mediates SOX17 Deficiency-Induced Pulmonary Hypertension

This study shows that SOX17 deficiency in endothelial cells causes pulmonary hypertension via E2F1 signaling, suggesting E2F1 as a therapeutic target in PAH.

TMEM100, a Lung-Specific Endothelium Gene

This study identifies Tmem100 as a lung-specific endothelial gene through single-cell RNA-sequencing analysis.

Loss of Endothelial HIF-Prolyl hydroxylase 2 (PHD2) Induces Cardiac Hypertrophy and Fibrosis

This study shows that loss of endothelial PHD2 causes cardiac hypertrophy and fibrosis via HIF-2, and PHD2 is reduced in human cardiomyopathy.

Endothelial PHD2 Deficiency Induces Nitrative Stress via Suppression of Caveolin-1 in Pulmonary Arterial Hypertension

This study shows that endothelial PHD2 deficiency leads to nitrative stress via suppression of Caveolin-1, contributing to pulmonary arterial hypertension and vascular remodeling.

Integrated characterization of SARS-CoV-2 genome, microbiome, antibiotic resistance and host response from single throat swabs

This study uses TRACE-seq to analyze single throat swabs, integrating SARS-CoV-2 genome, microbiome, antibiotic resistance, and host response data.

Base-resolution mapping reveals distinct m1A methylome in nuclear- and mitochondrial-encoded transcripts

The paper develops a base-resolution m1A profiling method and identifies distinct m1A methylome classes in human nuclear and mitochondrial transcripts, showing functional roles in translation efficiency and mitochondrial mRNA regulation.

A Critical-like Collective State Leads to Long-range Cell Communication in Dictyostelium discoideum Aggregation

The paper develops a multiscale model of Dictyostelium discoideum aggregation, showing that critical-like collective states enable long-range cell communication and optimal spore dispersal.