Designing and implementing programmable depletion in sequencing libraries with DASHit
D. Dynerman, A. Lyden, J. Quan, S. Caldera, A. McGeever, B. Dimitrov, R. King, G. Cirola, M. Tan, R. Sit, M. van den Berge, H. A. M. Kerstjens, A. Faiz, S. Christenson, C. Langelier, , E. D. Crawford
The paper introduces DASHit, a software tool that designs CRISPR-based depletion targets to reduce high-abundance sequences in sequencing libraries, demonstrating its effectiveness across three species.
bioRxiv · Jan 12, 2020 · PreprintMicrobiology